Cell and Tissue Imaging Core

细胞和组织成像核心A由一个集中的、尖端的显微镜设备组成，对于PO1申请中的4个项目的研究目标来说是绝对必要的，该申请的标题是“治疗肝纤维化和阿尔法1-AT缺乏症(ATD)的过度增殖的新疗法”。该设施位于匹兹堡大学医学中心主校区的生物成像中心(CBI)(www.cbi.bitt.edu)内。所有PI项目已经并将继续大量和多样化地使用CBI进行特定于设施的成像方法。在核心A(Stolz)的PI和/或生物成像中心的其他教职员工和单个项目的PI之间的合著出版物中可以看到这种使用的持久性证据。CBI提供的成像专业包括所有超微结构电子显微镜(透射电子显微镜、扫描电子显微镜、免疫电子扫描和透射电子显微镜、负染)、光学和荧光显微镜(宏观、解剖、光和荧光、表观荧光、共聚焦扫描和多光子成像)、活细胞显微镜(透射光和荧光)和高级荧光专业，如FRET；FRAP光谱分析和比率成像。对数据处理也至关重要的是，向方案调查人员及其实验室工作人员提供了广泛的图像分析、定量评估软件和技术援助。由于专业知识和设备位于一个集中的设施中，因此可以实现成像设备之间的无缝集成。此外，仪器可通过可编程钥匙卡访问，并由训练有素的用户全天候使用。受过训练的用户可以通过我们的在线调度网站注册时间，该网站也是PIS之间基于互联网的数据共享场所。

The Cell and Tissue Imaging Core A is comprised of a centralized, cutting-edge microscopy facility, absolutely essential to the research goals of the 4 Projects in this PO1 application entitled "New therapies for liver fibrosis and hyperproliferation in alpha1-AT deficiency (ATD)". The facility is housed within the Center for Biologic Imaging (CBI) (www.cbi.pitt.edu) at the main campus of the University of Pittsburgh Medical Center. All Project Pis have made, and continue to make, heavy and diverse use of the CBI for facility-specific imaging methodologies. Evidence of the longevity of this use is seen in co-authored publications between the PI of Core A (Stolz), and/or other faculty and staff members at the Center for Biologic Imaging and Pi's ofthe individual projects. The imaging specialties afforded by CBI include all ultrastructural electron microscopy (transmission electron microscopy, scanning electron microscopy, immuno-electron SEM and TEM, negative staining), light and fluorescence microscopy (macro, dissecting, light and fluorescence, epi-fluorescence, confocal scanning and multi-photon imaging), live cell microscopy (transmitted light and fluorescence) and advanced fluorescence specialties like FRET; FRAP spectral analysis and ratiometric imaging. Also critical to data processing, a wide range of image analysis, quantitative evaluation software and technical assistance is available to program investigators and their laboratory staff. Since the expertise and equipment is located in one centralized facility, seamless integration among imaging modalities can be realized. Furthermore, instrumentation is accessible via programmable key card and is available 24/7 by trained users. Trained users can sign up for time through our on-line scheduling website which also serves as an internet-based data sharing venue among Pis.