Signaling, Motility and TEM Assays

信令、动力和透射电子显微镜分析核心的目的是提供方法学、实验评估、 评估肿瘤和间质的个体行为的体外试验的分析和解释 细胞以及它们之间的相互作用。核心执行检测2D活动细胞行为的分析，3D 侵袭和跨内皮细胞迁移。这些分析允许对细胞行为进行完整的评估 通过血管内皮细胞的趋化性、侵袭和迁移，并提供对 在昂贵和耗时的动物研究之前，给出一种细胞类型的侵袭能力。此外，这些 体外测试支持和补充了侵入性多光子成像提供的体内研究 和瞬变电磁能力细胞收集和分析核心(核心A)。核心B还起到了程序化的作用 用于使用独特的蛋白质生物传感器和检测发明的形成的资源，因此 确保在所有项目中统一应用这些技术。化验结果支持 将允许对肿瘤和间质细胞所使用的分子机制进行尖锐的分析 促进肿瘤细胞侵袭、渗入和渗出。 相关性(请参阅说明)： 核心B提供的服务对于计划项目的总体目标至关重要，以定义 支持侵袭和转移的肿瘤和间质细胞之间的详细相互作用。这些是体外培养的 分析提供了关于细胞行为和机制的信息，这对于解释In 活体和活体成像研究。核心还协调使用独特的生化试剂， 由个别实验室开发的生物传感器和方法，以分发给所有项目。

The aim of the Signaling, Motility and TEM Assays Core is to provide methodology, experimental evaluation, analysis and interpretation of in vitro assays in order to assess the individual behavior of tumor and stromal cells as well as their interactions. The Core performs assays examining 2D motile cell behavior, 3D invasion, and transendothelial migration. These assays permit a complete evaluation of cell behavior during chemotaxis, invasion and migration across a vascular endothelium, and provide a thorough analysis of the invasive capabilities of a given cell type prior to costly and time-consuming animal studies. Moreover, these in vitro assays support and complement the in vivo studies provided by the Multiphoton Imaging, Invasive and TEM Competent Cell Collection and Analysis Core (Core A). Core B also functions as a programmatic resource for the use of unique protein biosensors and for assays examining invadopod formation, and thus ensures that the application of these technologies is uniform across all of the projects. The assays supported by the Core will permit an acute analysis of the molecular mechanisms used by tumor and stromal cells to promote tumor cell invasion, intravasation and extravasation. RELEVANCE (See instructions): Services provided by Core B are essential for the overall objectives of the Program Project to define the detailed interactions between tumor and stromal cells that support invasion and metastasis. These in vitro assays provide information about cellular behavior and mechanism that is essential for the interpretation of in vivo and intravital imaging studies. The Core also coordinates the use of unique biochemical reagents, biosensors and methodologies developed by individual laboratories for distribution to all of the projects.