Chemical Synthesis, Biochemistry & Spectroscopic Analysis

核心B是一个合成、分析和生化核心，将为这三个核心提供不可或缺的服务 项目核心B的合成组分将进行放大合成、纯化和分析， “工具包”和其他核苷酸类似物用于项目1和3。核心B的生化成分是 设计用于进行蛋白质纯化和合成项目所需的专门DNA结构 1和3.分析组件将负责获取和执行动力学的全局分析。 数据，包括前稳态停流荧光强度和旋转各向异性，快速猝灭 实验，以及基本的NMR和EPR-ENDOR分析，用于支持项目2和3。 核心B还将作为培养化学专业研究生和博士后学生的主要工具。 合成和蛋白质纯化技术，酶动力学分析的原理和实践， 实验NMR和EPR光谱技术。

Core B is a synthesis, analysis and biochemical core that will provide indispensable services for all three projects. The synthetic component of Core B will carry out scaled-up synthesis, purification and analysis of "tool-kit" and other nucleotide analogs for use in Projects 1 and 3. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DNA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy, rapid quench experiments, as well as basic NMR and EPR-ENDOR analysis, to be used in support of Projects 2 and 3. Core B will also serve as a primary vehicle for training graduate and postdoctoral students in chemical synthesis and protein purification techniques, the principles and practice of enzyme kinetic analysis, and techniques in experimental NMR and EPR spectroscopy.