Stanford Career Development Program in Omics of Lung Diseases

描述（由申请人提供）：斯坦福大学职业发展计划在肺部疾病组学（K12）。我们提出了一个职业发展计划（CDP）在肺部疾病组学的主要重点是肺动脉高压（PAH）。这是我们当前K12 CDP关于“肺部疾病的遗传学和基因组学”的逻辑扩展，重点关注PAH。新的CDP提案使我们能够为下一批MD和PhD科学家提供跨学科和生物信息学技能，以整合新的高通量基因组学，蛋白质组学和代谢组学平台，从而更好地了解疾病病理生理学。这个提议的组学CDP将推动我们通过NIH- NHLBI跨学科赠款建立的主要跨部门合作，重点是PAH。我们的一个NIH赞助的项目应用新的尖端高通量蛋白质组学方法来研究自身免疫性和特发性PAH中的炎症细胞。另一项研究利用基因组学和生物信息学以及诱导多能干细胞方法，将基因变异、甲基化变化和基因组中的RNA表达差异与PAH患者的天然和iPSC衍生内皮细胞的功能行为联系起来。我们在肺动脉高压突破倡议网络中的作用允许获得这些有价值的样本，我们在成人和儿童PAH方面的主要临床项目确保我们能够在这一重要研究领域培养新一代年轻科学家。我们的学者将拥有MD或PhD背景，我们的CDP在组学（基因组学，蛋白质组学和代谢组学）和计算生物学方面提供广泛的教学交叉培训，特别是对于在这些领域几乎没有背景准备的MD。此外，我们还为几乎没有接触过医学的博士学者提供肺血管解剖学，生理学和病理学方面的培训。每个学者将在培训的第一年开始进行一个指导研究项目，其中一名主要导师具有PAH，组学领域或生物信息学方面的专业知识，以及两名具有互补技能的共同导师。导师和共同导师将帮助学者获得这些技能，促进解释高通量研究产生的大型数据集，以揭示研究结果的生物学和临床相关性。我们的CDP专注于一种疾病，但同时为学者提供必要的工具，以应对未来高通量科学发现的挑战，改善许多其他肺部疾病的诊断和治疗。

DESCRIPTION (provided by applicant): Stanford Career Development Program in Omics of Lung Diseases (K12). We propose a Career Development Program (CDP) in Omics of lung diseases with a major focus on pulmonary arterial hypertension (PAH). This is a logical extension of our current K12 CDP on the "Genetics and Genomics of Lung Diseases" that focuses on PAH. The new CDP proposal allows us to equip the next cadre of MD and PhD scientists with interdisciplinary and bioinformatic skills to integrate new high throughput genomic, proteomic and metabolomic platforms to gain a better understanding of disease pathophysiology. This proposed CDP in Omics will fuel the major cross-departmental collaborations we have built through NIH- NHLBI Interdisciplinary grants focusing on PAH. One of our NIH sponsored projects applies novel cutting-edge high-throughput proteomic methodologies to investigate inflammatory cells in autoimmune and idiopathic PAH. Another utilizes genomics and bioinformatics and induced pluripotent stem cell methodology to relate gene variants, methylation changes and RNA expression differences across the genome, to the functional behavior of native and iPSC-derived endothelial cells from PAH patients. Our role in the Pulmonary Hypertension Breakthrough Initiative Network permits access to these valuable samples, and our major clinical programs in Adult and Pediatric PAH assure that we are well-positioned to train a new generation of young scientists in this important area of research. Our Scholars will have MD or PhD backgrounds and our CDP offers extensive didactic cross training in the omics (genomics, proteomics, and metabolomics) and in computational biology, particularly for the MD with little background preparation in these areas. As well, we offer training in lung vascular anatomy, physiology and pathology for the PhD Scholar with little exposure to medicine. Each Scholar will undertake a mentored research project beginning in the first year of training, with a primary Mentor who has expertise in PAH, in the Omics fields, or in Bioinformatics, and two Co-Mentors with the complementary skills. Mentors and Co-Mentors will help the Scholar attain those skills that facilitate interpreting the large data sets resulting fro high throughput research to uncover the biological and clinical relevance of the findings. Our CDP focuses on one disease but, at the same time, provides the Scholar with the necessary tools to take on the challenges of future high throughput scientific discovery, to improve diagnosis and treatment of many other lung diseases.