Light encoding properties of wiry-type and starburst amacrine cells of the primat

项目摘要 拟议研究的总体目标是确定光编码特性 灵长类动物视网膜中的两类无长突细胞：线状细胞和星状细胞。 尽管在细胞类型和电路的研究方面取得了巨大的进步 哺乳动物视网膜，基本上对无长突细胞在视网膜中的功能一无所知 灵长类视网膜。第一批录音将从钢丝型和星爆型进行 猕猴视网膜体外制备中的无长突细胞。 这项拟议的研究有两个具体目标： 1)确定丝状无长突细胞的光编码和固有特性。 灵长类动物的视网膜。我将对已鉴定的线状无长突细胞进行第一次记录 并使用运动刺激和时空白噪声来测量 这些单元格的编码属性。我将测试具体的假设(A)，即- 类型细胞更喜欢局部运动而不是全局运动，(B)电压门控钠通道 有助于它们的树突中的信号传播，以及(C)线状细胞是 伽巴气体。 2)确定灵长类星爆型无长突细胞是否具有方向选择性。我 将首次记录灵长类动物视网膜中已识别的星爆型无长突细胞 并使用运动刺激和空间噪声来直接确定灵长类恒星暴发 细胞具有方向选择性。我将具体测试灵长类动物的假设(A) 恒星暴增细胞表现出方向选择性，更喜欢离心式运动，以及(B) 灵长类动物和小鼠体内的星暴细胞表现出相似的光编码特性。

Project Summary The overall goal of the proposed research is to determine the light encoding properties of two classes of amacrine cell in the primate retina: wiry-type and starburst cells. Despite the tremendous advances in the study of cell types and circuits in the mammalian retina, essentially nothing is known of the function of amacrine cells in the primate retina. The first recordings will be performed from wiry-type and starburst amacrine cells in an in vitro preparation of the macaque monkey retina. The proposed research has two specific aims: 1) To determine the light encoding and intrinsic properties of wiry-type amacrine cells in the primate retina. I will make the first recordings of identified wiry-type amacrine cells in the primate retina and use motion stimuli and spatio-temporal white noise to measure the encoding properties of these cells. I will test the specific hypotheses (A) that wiry- type cells prefer local motion to global motion, (B) that voltage-gated sodium channels contribute to signal propagation in their dendrites, and (C) that wiry-type cells are GABAergic. 2) To determine whether primate starburst amacrine cells show direction selectivity. I will make the first recordings of identified starburst amacrine cells in the primate retina and use motion stimuli and spatial noise to determine directly whether primate starburst cells are direction selective. I will specifically test the hypotheses (A) that primate starburst cells show direction selectivity, preferring centrifugal motion and (B) that starburst cells in the primate and mouse show similar light encoding properties.