Large Animal Core

项目总结（见说明）： 大型动物中心将提供所需的设施，管理和技术专业知识，为拟议计划的所有项目提供MGH小型猪和狒狒和组织样本，以及专家兽医护理，免疫试剂和微生物诊断测定和支持。将分发临床样本，以最大限度地利用每只动物进行异种移植临床相关方案的研究。该计划每个组成部分的成功取决于该核心提供的动物、微生物支持和抗体的质量、可预测性和可获得性。 每周在主要研究者和兽医人员会议上审查动物护理。 大型动物中心的目标包括： 目标1：MGH MHC近交小型猪的生产、质量控制、跟踪和维持，包括具有α-1，3-半乳糖基转移酶基因的纯合破坏的新型MGH GalT-KO猪（参见图1和图2）和GalT-KO-CD 39转基因猪。小型猪的生产是使用一个计算机化的、基于网络的系统来监测的，该系统是为了协调每个项目的动物的育种、质量控制、分配、运输和利用而开发的。生猪生产的显著改善已实现了足够用于拟定研究的生猪供应的增加。 目标2：购买和饲养非人灵长类动物 目标3：为免疫功能低下的动物提供传染病/微生物学支持，为病毒病原体提供分子诊断检测，并就传染病管理提供建议。 目标4：与猪和狒狒细胞表面抗原反应的独特系列单克隆抗体（MAb）的生产、表征和维持，以评估猪和狒狒细胞谱系并改善细胞耗竭。 这些活动的协调工作由Scott Arn负责，他负责指导基因敲除猪的开发、灵长类动物的提供和MGH移植生物学研究中心所有研究人员使用的详细数据库的维护。Jay Fishman博士将负责异种移植研究中的传染病管理和微生物诊断。动物手术由经验丰富的移植外科医生、兽医临床医生和麻醉师在专用的无菌手术室进行，以优化护理和舒适度。

PROJECT SUMMARY (See instructions): The Large Animal Core will provide the facilities, management and technical expertise needed to supply MGH miniature swine and baboons and tissue samples for all projects of the proposed program as well as expert veterinary care, immunological reagents, and microbiologic diagnostic assays and support. Clinical samples will be distributed to maximize utilization of each animal for studies of clinically relevant regimens for xenotransplantation. The success of each component of this program is dependent on the quality, predictability, and accessibility of the animals, microbiologic support, and antibodies provided by this Core. Animal care is reviewed weekly at meetings of the principal investigators and the veterinary staff. The Aims of the Large Animal Core include: Aim 1: Production, quality control, tracking, and maintenance of MGH MHC inbred miniature swine including the novel MGH GalT-KO swine, with homozygous disruption of the a-1,3-galactosyltransferase gene (see Figures 1 and 2) and the GalT-KO-CD39 transgenic swine. Miniature swine production is monitored using a computerized, web-based system developed to coordinate the breeding, quality control, allocation, transportation and utilization of animals for each Project. Significant improvements in swine production have achieved an increased supply of swine adequate to the proposed studies. Aim 2: Purchase and maintenance of non-human primates Aim 3: Infectious Disease/Microbiology support for immunocompromised animals to provide molecular diagnostic assays for viral pathogens and advice on infectious disease management. Aim 4: Production, characterization, and maintenance of a unique series of monoclonal antibodies (MAb)reactive with swine and baboon cell surtace antigens to assess pig and baboon cell lineages and improve cellular depletion. Coordination of these activities is performed by Scott Arn, who directs all aspects of the development of the knockout swine, the provision of primates and maintenance of the detailed database used by all investigators of the Transplantation Biology Research Center at MGH. Dr. Jay Fishman will oversee infectious disease management and microbiologic diagnostics in xenotransplantation studies. Animal procedures are conducted by experienced transplant surgeons, veterinary clinicians and anesthesiologists in dedicated sterile operating rooms to optimize care and comfort.