INTEGRATIVE BIOLOGY

项目总结（见说明）： 整合生物学核心为德克萨斯医学中心消化疾病研究核心中心（DDC）的成员及其研究人员提供以下方面的培训和服务：1）胃肠（GI）道的动物手术; 2）利用体内和体外系统对GI特性进行生理学测量; 3）消化疾病动物模型的开发; 4）细胞分离; 5）整体动物成像技术，用于非侵入性地检测功能和解剖学异常，并监测生物标志物、药物和微生物的运输和分布。UTHSC的Lichtenberger和Uray博士在GI生理学和药理学领域具有专业知识，专门研究GI分泌、屏障功能和GI平滑肌功能的体内和体外模型，并在其实验室中开发了许多动物模型以研究消化系统疾病的病理生理学机制。Smith博士是一名合作者，他将为中心带来动物外科专业知识，并为研究需要动物处理和/或手术的中心成员提供帮助和培训，并将在与IRB批准实验室动物研究相关的问题上提供帮助。Thevananther博士将为DDC的成员提供有价值的服务，他们需要分离的啮齿动物肝细胞和枯否细胞用于培养或其他体外研究，枯否细胞分离是一项新的服务。我们还增加了一项新的服务，以帮助DDC成员在培养中生长和研究GI类器官。为DDC成员提供的另一项服务将是提供整体动物成像的帮助，以非侵入性地检测结构和功能的改变，并监测分子示踪剂的运动，分子示踪剂已成功用于监测GI运动和屏障特性。综合生物学核心旨在帮助中心成员开发利用实验室动物和组织实现其研究目标所需的技术。除董事外，经验丰富的动物生理和外科技术员将根据优先级计划为DDC成员提供实践培训，以确保初级研究者的发展。综合生物学核心的工作人员还将促进中心成员之间的互动和合作。

PROJECT SUMMARY (See instructions): The Integrative Biology Core provides training and service to the members ofthe Texas Medical Center Digestive Diseases Research Core Center (DDC) and their research staff in: 1) animal surgery of the gastrointestinal (Gl) tract; 2) physiological measurements of Gl properties utilizing both in vivo and in vitro systems; 3) the development of animal models of digestive diseases; 4) cell isolation; 5) whole animal imaging techniques to non-invasively detect functional and anatomical abnormalities, and to monitor the traffic and distribution of biomarkers, drugs and microorganisms. Drs. Lichtenberger and Uray at UTHSC have expertise in the field of Gl physiology and pharmacology, specializing in the in vivo and in vitro models of Gl secretion, barrier function and Gl smooth muscle function, and have developed a number of animal models in their laboratories to study pathophysiological mechanisms of digestive diseases. Dr. Smith, a Collaborator, will bring expertise in animal surgery to the Core and provide assistance and training to members ofthe Center whose research requires animal handling and/or surgery and will provide assistance in issues related to IRB approval for research on laboratory animals. Dr. Thevananther will provide a valuable serve to members of the DDC who require isolated rodent hepatocytes and Kupffer cells for culture or other in vitro studies, with Kupffer cell isolation being a new service. We have also added a new service to assist DDC members in the growth and study Gl organoids in culture. Another service offered to members of the DDC will be to provide assistance with whole animal imaging to non-invasively detect alterations in structure and function, and to monitor the movement of molecular tracers, which has been used successfully to monitor Gl motility and barrier properties. The Integrative Biology Core aims to assist the members ofthe Center in the development of the required techniques utilizing laboratory animals and tissues to accomplish their research goals. In addition to the Directors, the services of an experienced Animal Physiological & Surgical Technician will provide hands on training of members ofthe DDC in accordance to a prioritization schedule to assure the development of junior investigators. The staff of the Integrative Biology Core will also serve to facilitate interactions and collaborations between members ofthe Center.