Assigning function to Acinetobacter baumanii sRNAs

鲍曼不动杆菌是一种新出现的病原体， 感染.在伊拉克和阿富汗，它也给军队带来了问题， 与战争创伤有关的感染。A. baumannii Xhaf致力于 作为病原体的持久性是其积累耐药基因的倾向和惊人的能力， 耐受杀菌剂和干燥。该项目将使用RNA-seq分析来识别和定义功能 sRNA调节子（sRNA）的表达与七种临床相关的耐药性状有关， A.鲍曼不动杆菌。这些包括对四种抗生素，两种杀虫剂和干燥的耐药性。sRNAs是 它们对特定的环境信号作出反应，它们迅速合成，并迅速发挥作用， 在翻译水平控制基因表达。我们假设sRNAs帮助A.鲍曼尼-奥科科普 与抗生素、杀生物剂和干燥暴露相关的细胞被膜和代谢应激。我们 这些结果将为开发新的抗不动杆菌感染的防线开辟道路。

Acinetobacter baumannii is an emerging pathogen responsible for a significant percentage of nosocomial infections. It has also been problematic for the military in Iraq and Afghanistan, where it is the cause of softtissue infections associated with war-related trauma. Two properties of A. baumannii Xhaf contribute to its persistence as a pathogen are its propensity to accumulate drug resistance genes and a striking ability to tolerate biocides and desiccation. This project will use RNA-seq analysis to identify and define the functions of sRNA regulators (sRNAs) whose expression is associated with seven clinically relevant resistance traits of A. baumannii. These include resistance to four antibiotics, two biocides and desiccation. sRNAs are expressed in response to specific environmental signals, they are synthesized rapidly and they act quickly to control gene expression at the translational level. We hypothesize that sRNAs help A. baumannii io cope with cell envelope and metabolic stresses associated with antibiotic, biocide and desiccation exposure. Our results will open avenues for the development of new lines of defense against Acinetobacter infections.