CORE E: Tissue Culture

动物和人类的细胞系，特别是那些来自患者的细胞系，对于分离大量的 基因组DNA和RNA，以及制备蛋白质提取液，用于蛋白质印迹研究和酶 分析。可以从从全血中分离的淋巴细胞中提取基因组DNA。然而， 患者的永生化B淋巴母细胞或原代皮肤成纤维细胞的可用性特别高 出于几个原因，这是有利的。细胞系提供了一种可靠的方法来获得额外的DNA 在不打扰患者和家属的情况下进行额外的静脉穿刺术是必要的。这也是一种保障， 特别是在病态(致命)疾病的情况下，确保病机的DNA、RNA和蛋白质来源 进行研究，并在获得新的信息和技术时进行重复分析。此外，单元格 在涉及瞬时和长期表达的研究中，LINE用于电穿孔和病毒感染。 最后，来自IDD患者的成纤维细胞在未来可能被证明具有诱导多能性的价值。 机械学研究和潜在的治疗学筛查。

Animal and human cell lines, particulariy those derived from patients, are crucial for isolating large quantities of genomic DNA and RNA, and for preparing protein extracts for Western blotting studies as well as enzyme analysis. Genomic DNA can be prepared from lymphocytes isolated from whole blood. However, the availability of immortalized B-lymphoblasts or primary skin fibroblasts from the patient is particularly advantageous for several reasons. Cell lines provide a reliable means of securing additional DNA when necessary without disturbing the patient and family members in additional venipunctures. It is also a safeguard, especially in the case of morbid (fatal) disease, to ensure DNA, RNA and protein sources for pathogenesis studies and for repeated analysis when new information and technology become available. In addition, cell lines are used for electroporation and viral infections in studies involving transient and long term expression. Lastly, fibroblasts from patients with IDD are likely to prove valuable in the future for induction into pluripotent mechanistic studies and potentially for screening for therapeutics.