Pathology Core

1. 概述 NHLBI病理学核心设施提供了一个全方位的组织病理学服务的研究计划。我们的服务包括组织学组织程序，诊断组织病理学评估和组织学和免疫组织化学程序的培训。 核心服务： 第一章 病理学核心的主要服务是组织学程序，如福尔马林固定的组织包埋，切片和组织学染色。为此，核心接收固定或新鲜条件下的样品。对这些样本进行处理并包埋在石蜡或OCT组件中，用于石蜡或冷冻切片。石蜡包埋的样品可以常规切割为5微米厚的切片，固定在载玻片上。整个器官或胚胎的连续切片，或厚（20-100微米）和薄（5-10微米）阶梯切片的混合切片也可以根据要求切割。冷冻切片通常以8-10微米的厚度切割。病理学核心根据Harris方法（组织技术实验室方法）使用苏木精和伊红进行常规组织学染色。Armed Forces Institute of Pathology，Edited by Prophet EB，米尔斯B，Arrington JB and Sobin LH，pp 56，American Registry of Pathology，华盛顿，DC，1992）。此外，我们还进行了特殊的组织学染色，包括PAS、Masson三色、Movat、Elastic货车Van Gieson和油红O染色。所有这些服务都由核心员工执行，并及时完成。大多数请求都在两周内完成，但紧急请求除外，紧急请求可以安排为较高优先级的请求。在某些情况下，结果可以在第二天返回给用户。 （二） 诊断病理学。对于这项服务，核心负责人参与讨论实验设计，样品收集方法和结果的最终评估。我们提供组织学产品，如切片和染色的组织学切片，执行免疫染色程序，评估结果，描述结果，并制作具有代表性照片的评分表。在大多数情况下，这项服务将有助于稿件提交，我们将提供最终的显微照片。 第三章 为组织病理学和免疫组织化学领域的专家和研究员提供培训、咨询和解决问题。我们提供石蜡和冷冻样本的组织处理、包埋和切片培训。此外，我们还提供抗体测试和免疫染色程序以及免疫染色问题咨询。 2.运行分析 自我们上次报告以来，病理学中心共收到285份上述服务请求。核心用户包括来自27个PI/实验室的58名研究员/医生。我们总共处理了2，747个组织块，并切割了22，865个组织学切片，这些切片通常安装有2或3个组织切片。其中，有4，301张切片用苏木精和伊红染色用于常规组织学。此外，我们还进行了1，250张载玻片，用于上述其他特定染色程序。 用户和服务的详细列表显示在所附的PDF文件中，该文件由iLab系统生成。表格显示的数量包括所有载玻片、组织块和染色。 核心负责人参与组织病理学分析服务，并为来自6个PI/实验室的11名科学家的研究项目进行免疫染色，包括组织病理学诊断，评分系统的组织学评价，并为出版物提供最终图像。2013-2014年，这些出版物共计10份。 病理学核心提供冷冻/石蜡切片切割，免疫组织化学程序和组织学显微照片使用核心明场显微镜装备数码相机的培训。核心负责人与科学家密切合作，选择抗体和方案，然后提供动手染色演示，直到科学家能够独立执行程序。目前，我们有18个用户使用Leica DMB-4000显微镜，5个用户使用Cryo-stat（冷冻切片）

1. Overview The NHLBI Pathology Core Facility provides a full range of histopathological services for the DIR program. Our services include histology tissue procedures, diagnostic histo-pathology evaluation and training for histology and immuno-histo-chemistry procedures. Core Services: 1) The main services of the Pathology Core are histology procedures, such as formalin-fixed tissue embedding, sectioning and histology staining. For this purpose, the Core receives samples in fixed or in fresh condition. These samples are processed and embedded in paraffin or OCT component for either paraffin or frozen sections. The paraffin embedded samples can be cut routinely as 5 micron thick sections mounted on glass slides. The serial sections through an entire organ or embryo, or mixed with thick (20-100 microns) and thin (5-10 microns) step sections also can be cut upon request. Frozen sections are normally cut at a thickness of 8-10 microns. The Pathology core provides routine histology staining with Hematoxylin and Eosin according to Harris methods (Laboratory Methods in Histotechnology. Armed Forces Institute of Pathology, Edited by Prophet EB, Mills B, Arrington JB and Sobin LH, pp 56, American Registry of Pathology, Washington, DC, 1992). In addition, we also perform special histology staining, including PAS, Masson tri-chrome, Movat, Elastic Van Gieson and Oil-Red O stain. All these services are performed by the Core staff and finished in a timely manner. Most requests are completed within a two-week period, except urgent requests that can be arranged as a higher-priority request. In some cases, results can be returned to users the next day. 2) Diagnostic pathology. For this service, Core head is involved in discussion of experimental design, sample collection methods and final evaluations of the results. We provide histology products such as cut and stained histology slides, perform immune-staining procedures, evaluate the results, describe the findings and make a scoring sheet with representative photographs. In most cases, this service will contribute to manuscript submission and we will provide final microscopic figures. 3) Training, consultation and trouble shooting for DIR scientists and fellows in the fields of histo-pathology and immuno-histochemistry. We provide training for tissue processing, embedding and sectioning for paraffin and frozen samples. In addition, we also offer testing for antibodies and for immuno-staining procedures and consultation for immuno-staining problems. 2. Operational analysis Since our last report, the pathology core had total of 285 requests for the services listed above. Core users include 58 fellows/doctors from 27 PIs/ laboratories. We have processed a total of 2,747 tissue blocks and cut 22,865 histology slides, which are usually mounted with 2 or 3 tissue sections. Among them, there were 4,301 slides stained with Hematoxylin and Eosin for routine histology. In addition, we also performed 1,250 slides for other specific staining procedures as mentioned above. Detailed lists of user and services are shown in the attached PDF file, which is generated by iLab system. The quantity of table showed included all slides, blocks and staining. Core head is involved in the services for histo-pathological analysis, and immunostaining on research projects for 11 scientists from 6 PI/laboratories including histo-pathological diagnosis, histology evaluations with the score system and provided final images for publications. These resulted in 10 publications in 2013-2014. The Pathology Core offers training for cutting frozen/paraffin sections, immunohistochemistry procedures and histology microphotograph by using Core bright field microscopy equipped digital camera. Core head worked closely with the scientists for selection of antibodies and protocols, and then provided hands-on staining demonstration until the scientist can perform the procedures independently. Currently, we have 18 users for Leica DMB-4000 microscopy and 5 for Cryo-stat (Frozen sections)