Intravital Microscopy Core

核心领导人：托尔斯滕·R·门佩尔。活体显微镜核心(核心A)是根据U19研究人员的明确需求制定的 旨在借鉴其关键人员在多光子体内的丰富经验 显微镜(MP-IVM)技术，提供专业知识和专门设备，并协助设计和 进行外源RNA和EV介导的胶质母细胞瘤与其细胞间通讯的体内成像研究 间质环境和周围健康的脑组织。 活体显微镜核心的具体目标如下： 1.在利用多光子显微镜技术设计具体实验方面提供专业知识 胶质母细胞瘤EV的生物生成、胞外特性及非恶性摄取的分析 脑细胞，以及功能性exRNA和EV介导的细胞间通讯 肿瘤细胞和肿瘤间质。 2.提供对所需仪器的访问，并协助执行MP-IVM实验 使用PIS项目在核心帮助下开发的胶质母细胞瘤小鼠模型 目标1。 3.提供计算资源，用于存储、处理、深入分析和解释 在AIM 2中生成的数字成像数据。

Core Leader: Thorsten R. Mempel. The Intravital Microscopy Core (Core A) has been formulated based on a stated need of U19 investigators and is designed to draw on the considerable experience of its key personnel in multiphoton intravital microscopy (MP-IVM) techniques to provide expertise and specialized equipment and aid in the design and execution of in vivo imaging studies of exRNA- and EV-mediated communication of glioblastomata with their stromal environment and the surrounding, healthy brain tissue. The Specific Aims ofthe Intravital Microscopy Core are as follows: 1. To provide expertise in the design of specific experiments utilizing multi-photon microscopy-based analysis of EV biogenesis by glioblastomata, their properties in the extracellular space and uptake by nonmalignant brain cells, as well as of functional exRNA- and EV-mediated intercellular communication between tumor cells and tumor stroma. 2. To provide access to the required instrumentation and to aid in the execution of MP-IVM experiments using mouse models of glioblastoma developed by the project Pis with assistance of the core as outlined in aim 1. 3. To provide computational resources forthe storage, processing, in-depth analysis, and interpretation of digital imaging data generated in aim 2.