Temperature Compensation of Neuronal and Network Function

描述（由申请人提供）：温度是一种全局扰动，或多或少地改变所有生物反应和过程。这个提议解决了一个基本问题，即当引起网络动力学的所有突触和内在特性都因温度而发生不同变化时，作为行为基础的神经元回路如何能够对温度变化保持鲁棒性。为了实现这一目标，实验和计算研究的甲壳动物中央模式生成网络，幽门节律的口胃神经节（STG）的建议。具体目标包括：研究了温度对适应不同温度的动物的幽门回路单个神经元和突触的影响;温度对适应不同温度的动物的STG神经元的六种电压和时间依赖性膜电流的影响的电压钳特性;温度对由分离的STG神经元对构成的相互抑制回路的动态钳的影响;开发基于电导的单神经元模型;以及构建幽门网络模型，以探索电路性能对温度变化的鲁棒性。这些数据将使我们了解如何在个体之间保持稳健的电路性能，以及如何响应电路组件随时间的变化。这些实验将有助于我们理解具有不同底层电路组件的个体如何能够对许多环境扰动做出稳健的反应。此外，这些实验将提供对高温可能导致癫痫发作和其他神经问题的机制的深入了解。

DESCRIPTION (provided by applicant): Temperature is a global perturbation that alters all biological reactions and processes to a greater or lesser degree. This proposal address the fundamental question of how neuronal circuits that underly behavior can be robust against temperature changes when all of the synaptic and intrinsic properties that give rise to network dynamics are altered differently by temperature. Towards this goal, experimental and computational studies of a crustacean central pattern generating network, the pyloric rhythm of the stomatogastric ganglion (STG) are proposed. Specific Aims include: studies of the effects of temperature on individual neurons and synapses of the pyloric circuit from animals acclimated to different temperatures; voltage-clamp characterization of the effects of temperature on six voltage and time-dependent membrane currents from STG neurons from animals acclimated to different temperatures; the effects of temperature on dynamic clamp constructed reciprocal inhibitory circuits made from pairs of isolated STG neurons; development of conductance-based single neuron models; and construction of pyloric network models to explore the robustness of circuit performance to altered temperature. These data will inform our understanding of how robust circuit performance is preserved across individuals and in response to alterations in the components of a circuit over time. These experiments will contribute to our understanding of how individuals with different sets of underlying circuit components can nonetheless respond robustly to many environmental perturbations. Additionally, these experiments will provide insight into the mechanisms by which high temperature may contribute to seizure and other neurological problems.