UC Davis Shared Astrios Cell Sorter

 描述(由申请人提供)：加州大学戴维斯分校(UCD)是一所多元化的教育机构，在医学、兽医和农业研究方面表现出色。1998年，UCD流式细胞术共享资源实验室获得了S10计划支持(S10-RR012964-01)，购买了一台多激光、10色细胞分裂MoFlo高速细胞分选机。MoFlo细胞分选机设计的特定功能非常适合在共享资源实验室中使用，便于分析和分选来自人类和动物组织的各种细胞，以及微粒、微生物和植物细胞原生质体。15年来，MoFlo一直在戴维斯和萨克拉门托的UCD医学中心，包括医学院和兽医学院、NCI综合癌症中心和加州国家灵长类动物研究中心，支持UCD研究人员在广泛的多学科项目中的研究。然而，在多年的可靠服务之后，最初的“传统”MoFlo现在需要更换了。关键部件系统陈旧过时，变得不可靠，维护起来昂贵或不可能，尤其是水冷气体激光器。此外，传统MoFlo的大尺寸不允许将其封闭在生物安全密封柜中，也不符合国际细胞学促进学会的现代生物安全建议和新批准的NIH细胞分选机生物安全政策。拟议的替代仪器Beckman Coulter AstriosEQ提供了现代组件，但使用了同样稳定、可靠的流体设计，这是MoFlo细胞分选机的标志。AstriosEQ将配备4个激光器和16个光电探测器来检测常用的荧光探针和蛋白质，并将安装在具有气雾剂管理的生物安全安全罩中，以确保对生物有害样本的安全分类。加州大学戴维斯分校NIH支持的生物学研究的成功取决于灵活、可靠和安全的细胞分选机的不间断可用性。AstriosEQ将由戴维斯园区共享流式细胞仪实验室容纳、管理和维护，以便UCD的调查人员在未来几年能够获得细胞分选仪器和流式细胞仪专业知识。

 DESCRIPTION (provided by applicant): The University of California at Davis (UCD) is a diverse educational institution recognized for excellence in medical, veterinary and agricultural research. In 1998, the UCD Flow Cytometry Shared Resource Laboratory received S10 program support (S10-RR012964-01) to purchase a multi-laser, 10-color Cytomation MoFlo high- speed cell sorter. Specific features in the design of the MoFlo cell sorter are ideally suite for use in a shared resource lab, facilitating the analysis and sorting of a variety of cells from human and animal tissues, as well as microparticles, microorganisms, and plant cell protoplasts. For 15 years, the MoFlo has supported the research of UCD investigators across a wide range of multidisciplinary programs in Davis and at the UCD Medical Center in Sacramento, including the Schools of Medicine and Veterinary Medicine, NCI Comprehensive Cancer Center and the California National Primate Research Center. However, after years of reliable service, the original "legacy" MoFlo is now in need of replacement. Key component systems are aged and obsolete, have become unreliable and are expensive or impossible to maintain, particularly the water-cooled gas lasers. Further, the large size of the legacy MoFlo does not allow for enclosure in a biosafety containment cabinet and fails to meet the modern biosafety recommendations of the International Society for the Advancement of Cytometry and the newly approved NIH Biosafety Policy for Cell Sorters. The proposed replacement instrumentation, the Beckman Coulter AstriosEQ, offers modern components but uses the same stable, reliable fluidic design that is the hallmark of MoFlo cell sorters. The AstriosEQ will be equipped with 4 lasers and 16 photodetectors to detect commonly used fluorescent probes and proteins and will be housed in a biosafety containment hood with aerosol management to ensure safe sorting of biohazardous samples. The success of NIH supported biological research at UC Davis depends on the uninterrupted availability of a flexible, reliable, and safe cell sorter. The AstriosEQ willbe housed in, managed and maintained by the Davis campus Shared Flow Cytometry Laboratory so that UCD investigators have access to cell sorting instrumentation and flow cytometry expertise for years to come.