Electrophysiology

核心F-电生理学-玛丽-帕斯卡勒·科特博士 分子和组织学技术通常被用来评估治疗干预是否成功 促进脊髓损伤后轴突再生，影响脊髓可塑性。这些技术的局限性在于 他们无法确认这些功能调制的变化(即传导)的有效翻译 具体途径和/或影响Netia/ork活动)。再生的轴突已经延伸到 某一点并不一定意味着它们形成突触，更不用说它们是有效的和 功能齐全。虽然行为测试解决了这个问题的一部分，但个体间的可变性可能会带来 数据解释的复杂性，并且没有提供关于哪个小区/网络负责的细节 感觉运动系统的功能恢复。电生理核心的目标是提供更多 在实验动物亚群中进行复杂和详细的功能测试，更具体地说， 为没有专业知识/设备进行这类研究的实验室提供援助 实验 电生理学核心为实验设计提供帮助，并监督工作人员的培训 针对其个人需求量身定做的单个项目。核心保证了程序的统一性 给出了测试范例以及跨项目的生理测试和方法。核心还包括 开发和维护用于分析的有用的测试仪器、程序和软件。 这一核心的主要重点是培训个人评估使用脊髓损伤后干预的有效性 适当的电生理方法。处长将协助首席调查员 确定合适的实验设计并培训项目人员以执行此测试培训将 包括给定协议所需设备的操作以及执行程序、收集 数据和分析数据。核心还根据需要提供解释数据的协助。

CORE F - Electrophysiology - Marie-Pascale Cote, Ph.D. - PI Molecular and histology techniques are routinely used to assess if therapeutic interventions successfully enhance axonal regeneration and impact spinal plasticity after SCI. A limitation ofthese techniques relies in their inability to confirm an effective translation ofthese changes in functional modulation (i.e. conduction in specific pathways and/or influence netiA/ork activity). Regenerated axons that have extended beyond a certain point does not necessarily imply that they form synapses, much less that they are effective and functional. Although behavioral testing addresses part of this issue, inter-individual variability can introduce complexity in the interpretation of data and does not give details about which cell/network is responsible for recovered function in sensorimotor systems. The goal of the Electrophysiological Core is to provide more sophisticated and detailed testing of function in subsets of experimental animals and more specifically to provide assistance to laboratories that do not have the expertise/equipment to carry out this type of experiment The electrophyhsiological core offers assistance in experimental design and oversees training of staff from individual projects that is tailored to their individual needs. The core assures unformity of procedures for a given testing paradigm and access to physiological testing and methods across projects. The core also develops and maintains useful test apparatus, procedures and software for analysis. The primary focus of this Core is to train individuals to evaluate the efficacy of interventions after SCI using the appropriate electrophysiological methods. The Director will assist the Principal Investigator in determining a suitable experimental design and train Project personnel to perform this test Training will include the operation of devices required for a given protocol as well as performing the procedure, collecting data and analyzing data. The Core also offers assistance to interpret data as needed.