OUHSC Macromolecular X-ray Facility

OUHSC X射线核心设施（1998年由NIH授予Paul Weigel博士的IDEA资助启动）提供 专业知识和设备的各个COBRE项目的各种关键技术， 蛋白质和核酸的晶体学和小角X射线散射研究（SAXS）。的 技术包括在室温下筛选晶体的衍射质量，晶体冷冻保护剂 筛选，衍射数据收集，衍射数据处理，空间群归属，结构 测定、结构细化、结构分析和图形制备。核心设施将是 升级为包括SAXS仪器和所需的X射线发生器升级， 可用于水晶工作的端口。新发电机的光子通量将增加四倍， 直径梁。这两个特征对于筛选常见的小的、弱衍射的晶体是至关重要的。 COBRE研究项目X射线设施主任监督仪器的维护， 安排用户，并为Jls、其他COBRE培训SAXS和X射线晶体学用户 成员和OSBN成员。核心设施将由Blaine Mooers博士指导， 奥根·皮奥斯扎克博士和富兰克林·海斯博士的邀请他们有超过35年的 在大分子晶体学方面的经验。

The OUHSC X-ray Core Facility (initiated in 1998 with a NIH IDeA grant to Dr. Paul Weigel) provides expertise and equipment to the individual COBRE projects on a variety of techniques critical to crystallographic and small angle X-ray scattering studies (SAXS) of proteins and nucleic adds. The techniques include screening crystals for diffraction quality at room temperature, crystal cryo-protectant screening, diffraction data collection, diffraction data processing, space group assignment, structure determination, structure refinement, structure analysis, and figure preparation. The Core Facility will be upgraded to Include a SAXS instrument and the required X-ray generator upgrade which will have a second port available for crystal work. The new generator will have a fourfold greater flux of photons and a smaller diameter beam. Both features are critical for the screening the small, weakly diffracting crystals common in the COBRE research projects. The X-ray Facility Director oversees maintenance of the instruments, scheduling of users, and training users in both SAXS and X-ray crystallography for Jls, other COBRE members, and OSBN members. The Core Facility will be directed by Dr. Blaine Mooers with assistance from co-Directors Dr. Augen Pioszak and Dr. Franklin Hays. They have over 35 years of combined experience in macromolecular crystallography.