PHARMACOLOGY CORE

目的：药理学共享资源支持科罗拉多大学癌症中心(UCCC)成员的基础和临床研究项目的药代动力学(PK)研究规划和分析。 服务和技术：提供的服务包括：(1)PK研究设计咨询；(2)开发、验证和实施适用于生物液和组织中药物的分析分析；以及(3)分析数据的PK建模和数学分析。药理学共享资源采用的定量分析方法包括UV/Vis和荧光检测高效液相色谱(LC)串联质谱仪，以及其他生化方法(即酶联免疫吸附试验、酶联免疫吸附试验)。PK建模服务包括基于系统的方法(非分区建模)、分区建模、基于生理和基于人群的分析。药理学共享资源包括两台ABI3200线性捕捉器三重四极杆仪器，带有LC系统(Agilent 1200或岛津LC20)和HTC-PAL自动进样器，岛津显著LC系统具有UV/Vis(SPD-M20A)和荧光检测(RF-IOAxl)和冷藏自动进样器(S1L20AC)。会诊：药理学共享资源提供 咨询，帮助几乎没有药理学或PK背景的成员设计合适的实验和分析PK数据。使用：开发治疗计划中的癌症中心成员是药理学共享资源的主要用户；然而，激素相关恶性肿瘤、肺癌、头颈癌和癌症预防与控制计划的成员也是经常使用该资源的用户。药理学共享资源于2007年从安舒茨医学院转移到CSU，从那时起，UCCC成员的使用量每年都在增加，而非UCCC成员的使用量一直保持不变。自2005年最后一次竞争申请以来，药理学共享资源已经支持了来自联盟机构的39名UCCC调查人员和24名非UCCC成员。在过去的两年中，UCCC成员的14个研究项目和10个临床方案得到了支持。管理和财务：该资源由UCCC管理。目前，73.5%的运营预算来自对UCCC成员的收费，这些成员占设施用户的62%。这个 药理学共享资源请求为其运营预算的34%提供11.5万美元的CCSG支持。

Objective: The Pharmacology Shared Resource supports pharmacokinetic (PK) study planning and analysis for both basic and clinical research projects of University of Colorado Cancer Center (UCCC) members. Services and Technologies: Services offered include: (1) PK study design consultation; (2) Development, validation and implementation of appropriate analytical assays for drugs in biological fluids and tissues; and (3) PK modeling and mathematical analysis of analytical data. Quantitative analytical methods employed by the Pharmacology Shared Resource include UV/Vis and fluorescent detection HPLC, LC tandem mass spectrometry, and other biochemical methodologies (i.e. ELISA, enzymatic). PK modeling services include systems-based approaches (non-compartmental modeling), compartmental modeling, physiologically-based and population-based analyses. The Pharmacology Shared Resource, houses two ABI3200 linear trap triple quadropole instruments with LC systems (Agilent 1200 or Shimadzu LC20) and HTC-PAL autosamplers, a Shimadzu Prominence LC system with UV/Vis (SPD-M20A) and fluorescence detection (RF-IOAxl) and refrigerated autosampler (S1L20AC). Consultation: The Pharmacology Shared Resource provides consultation to help members with little pharmacology or PK background to design appropriate experiments and analyze PK data. Utilization: Cancer Center members in the Developmental Therapeutics Program are the primary users of the Pharmacology Shared Resource; however, members of the Hormone Related Malignancies, Lung, Head & Neck Cancer and Cancer Prevention & Control Programs are also regular users. The Pharmacology Shared Resource moved to CSU from the Anschutz Medical Campus in 2007 and since then, utilization by UCCC members has increased annually while use by non-UCCC members has remained constant. Since the last competitive application in 2005, the Pharmacology Shared Resource has supported 39 UCCC investigators from across the consortium institutions and 24 non-UCCC members. In the last two years, 14 research projects and 10 clinical protocols from UCCC members have been supported. Management and Finances: This resource is UCCC-managed. Currently, 73.5% of the operating budget comes from charge backs to UCCC members who represent 62% of facility users. The Pharmacology Shared Resource requests $115K CCSG support for 34% of its operating budget.