LiquidCell EM System with Temperature Control for Biological Imaging
LiquidCell EM System with Temperature Control for Biological Imaging
用于生物成像的带温度控制的 LiquidCell EM 系统
批准号：
8779700
负责人：
MingQiang Yi
金额：
$14.82万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
项目状态：
已结题
起止时间：
2014-09-15 至 2016-06-14
关键词：
BiologicalBiological ProcessBiological SciencesBiological TestingBiomedical ResearchBiophysicsCell physiologyCellsCellular biologyChemistryDevelopmentElectron MicroscopyEnvironmentFutureImageIndividualInvestigationJournalsLeadLifeLiquid substanceMarketingMembraneMicrofluidic MicrochipsMicrofluidicsMicroscopyMolecularNeurosciencesPhasePhysiologicalPrincipal InvestigatorProcessProteinsReactionResolutionRewardsSamplingSchemeScienceSmall Business Innovation Research GrantStressStructureSurfaceSystemTechniquesTechnologyTemperatureTestingThickTransmission Electron MicroscopyWaterWorkaqueousbiological researchbiological systemscancer therapycell assemblychemical reactioncolloidal nanoparticlecommercializationdesignexperiencein vivoinnovationlight microscopymacromoleculemembernanometernovelpublic health relevanceresearch studysilicon nitridesuccesstoolultra high resolutionvirology
中文摘要
翻译
描述（由申请人提供）：生物研究中最具挑战性但回报最高的任务之一是以高空间分辨率可视化分子机器的结构及其在生理条件下（例如在37 ℃的水环境中）在细胞功能中的相互作用。这种具有精确温度控制的LiquidCell EM系统能够使用透射电子显微镜以超高分辨率在真正的原生环境中直接成像许多动态生物过程。在这个方案中，我们将制作具有超薄观察窗的独立液体电池。37+/-0.1 ℃的精确温度控制将通过微流体装置制造实现。将进行单个蛋白质成像、蛋白质组装和活细胞功能的测试实验，以优化系统在生理条件下的体内生物成像。
英文摘要
DESCRIPTION (provided by applicant): One of the most challenging, yet highly rewarding tasks in biological research is to visualize with high spatial resolution the structure of molecula machines and their interactions in cellular function under physiological conditions, such as in 37 ¿C aqueous environment. This proposed LiquidCell EM system with precise temperature control enables direct imaging of many dynamic biological processes in truly native environments with ultra high resolution using transmission electron microscopy. In this proposal, we will fabricate the self-contained liquid cells with ultra-thin viewing windows. Precise temperature control of 37+/-0.1 ¿C will be achieved through microfluidic device fabrication. Test experiments of imaging individual proteins, protein assembly and live cell functions will be performed to optimize the system for in vivo biological imaging under physiological conditions.
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