Flow Cytometry and Cell Separation Shared Resource (FC-SR)

流式细胞术和细胞分离共享资源： 项目摘要 生物学共享资源组 普渡流式细胞术和细胞分离共享资源（FC-SR）提供先进的细胞和 粒子分析和分类能力，普渡大学癌症研究中心（PCCR）的成员。 作为一种分析工具，流式细胞术允许用户识别和定量细胞特征，细胞器，或 使用光学测量的结构部件。当与排序功能相结合时， 鉴定和分离具有特定性质或表达特定分子的单细胞，然后 进行进一步的检查和鉴定。在癌症研究中， 具有癌样特征的独特、特异性细胞是至关重要的，流式细胞术和细胞分选是极其重要的。 对当今的癌症研究者来说至关重要和有用。普渡大学的FC-SR提供广泛的服务， 支持24普渡癌症研究中心（PCCR）研究人员的研究项目，从所有四个 PCCR今年的研究计划。 FC-SR位于探索公园的宾德利生物科学中心（BBC）， 自2009年3月全面重组以来，其技术专长和仪器能力都得到了加强。一个主要 该设施的重点是在BSL-2条件下对小鼠和人细胞进行活细胞分选。在2010年与 在NIH S10共享仪器基金的支持下，FC-SR获得了高通量LEAP图像 扫描细胞仪该仪器为通过激光消融分选附着细胞增加了额外的维度 并用作流式细胞术和图像分析能力之间的桥梁。FC-SR还运行一个 作为分析和细胞分选的主要工具的FACS Aria III分选仪。目前，该设施 还操作FC 500细胞分析仪进行常规细胞分析。该设施的数据分析能力具有 随着许多FlowJo站点许可证的增加和新的高级数据的开发， 分析能力。 也许FC-SR提供的最重要的资源是工作人员分享的专业知识。许多 FC-SR的客户几乎没有进行细胞仪分析或设计实验的经验， BD FACS Aria III细胞分选仪。在没有FC-SR及其经验丰富的工作人员的情况下，这些调查人员 将在产生竞争性外部资金所需的细胞计数数据方面遇到巨大困难。

Flow Cytometry and Cell Separation Shared Resource: Project Summary Biology Shared Resource Group The Purdue Flow Cytometry and Cell Separation Shared Resource (FC-SR) provides advanced cell and particle analysis, and sorting capabilities to Purdue University Center for Cancer Research (PCCR) members. As an analytical tool, flow cytometry allows users to identify and quantitate cellular features, organelles, or structural components using optical measures. When combined with sorting capabilities, it is possible to identify and isolate single cells that have specific properties or express specific molecules and which can then be further examined and characterized. In cancer research, where the identification and characterization of unique, specific cells with cancer-like characteristics is critical, flow cytometry and cell sorting are extremely vital and useful to today's cancer researcher. Purdue's FC-SR provides a broad range of services that supported 24 Purdue Center for Cancer Research (PCCR) investigators' research projects from all four of PCCR's Research Programs in this current year. The FC-SR, located in the Bindley Bioscience Center (BBC) of Discovery Park, has grown considerably in both its technical expertise and instrument capabilities since its total re-organization in March 2009. A major emphasis of the facility is live cell sorting of mouse and human cells under BSL-2 conditions. In 2010 with support from an NIH S10 Shared Instrument Grant, the FC-SR obtained a high-throughput LEAP image scanning cytometer. This instrument adds an additional dimension to sorting of attached cells by laser ablation and serves as a bridge between flow cytometry and image analysis capabilities. The FC-SR also operates a FACS Aria III Sorter that serves as the primary workhorse for analysis and cell sorting. Currently, the facilty also operates a FC500 Cell Analyzer for routine cell analyses. The data analysis capabilities of the facility have advanced greatly with the addition of many FlowJo site licenses and the development of new advanced data analysis capabilities. Perhaps the most important resource provided by the FC-SR is the expertise that the staff shares. Many of FC-SR's clients have little, if any, experience conducting cytometry analysis or designing experiments using the BD FACS Aria III cell sorter. In the absence of the FC-SR and its experienced staff, these investigators would have tremendous difficulty in generating cytometry data necessary for competitive external funding.