An Orthogonal and Reversible Protein Labeling via Phosphopantetheinylation

 描述（由申请人提供）：蛋白质标记方法的进步是加速高含量显微镜技术、加快蛋白质组学分析以及扩展我们识别和监测疾病的工具的关键。该计划研究了一个可逆系统的发展，以标记选定的蛋白质。与传统方法不同，所提出的系统采用肽筛选技术作为工具，在两阶段酶对反应中编码离散选择性。选择性标记取决于标记酶的选择。所提出的计划开始通过计算分析正交标签的合理发展。该系统的评估包括详细的动力学分析和应用程序，了解蛋白质复合物。总的来说，该系统为蛋白质科学开辟了新的途径。该计划将提供一种新的方法，用于快速标记复杂的蛋白质纲要中的特定蛋白质，以用于细胞或组织内的未来研究。

 DESCRIPTION (provided by applicant): The advancement of protein labeling methodology is key to accelerating high-content microscopy technologies, expediting proteomic analyses, and expanding our tools to identify and monitor disease. This program examines the development of a reversible system to label selected proteins. Unlike conventional approaches, the proposed system adapts peptide-screening technologies as a tool to encode discrete selectivity within a two-stage enzymatic pair reaction. Selective labeling is dictated by the choice of labeling enzymes. The proposed program begins through the rational development of orthogonal tags by computational analysis. Evaluation of this system includes detailed kinetic analyses and applications for understanding protein complexes. Overall, this system opens new avenues in protein science. This program will provide a new method for rapidly labeling specific proteins within the complex compendium of proteins towards future studies within the cell or tissue.