Dako Omnis Autostainer for Screening Tumor Tissues with Immunohistochemistry

 描述（由申请人提供）：Roswell Park癌症研究所（RPCI）寻求资金购买免疫组织化学（IHC）用Dako Omnis自动染色仪。该仪器将存放在病理资源网络机构（PRN）中，并由Angela Omilian博士监督。PRN由癌症中心支持补助金支持，并已作为RPCI的正式共享资源核心为研究人员服务7年。与PRN机构相关的组织学专业知识可以追溯到更早的时候;该机构自2001年以来一直协助研究者进行自动化IHC。因此，PRN员工经验丰富，非常适合操作和维护所需设备。在典型的一年中，PRN处理至少7500个研究IHC载玻片，每天使用两台自动染色机来满足这一需求。最近，一台机器出现故障，其使用寿命即将结束。这是我们申请的动力。IHC是一种简单、成熟且具有成本效益的平台，用于检测人体组织中的蛋白质表达。在研究环境中，IHC通常用于筛选肿瘤组织中可用于癌症诊断、预后、分期和/或作为治疗干预标志物的生物标志物。通过Dako Omnis进行的自动化IHC实现了从运行到运行、质量的前所未有的标准化水平，还允许PRN机构每个工作日处理多达120个研究切片，轻松满足RPCI多名研究者的需求。该申请包括16名主要研究人员，他们跨越了基础和转化研究的不同领域，但都分享了RPCI的共同使命：“了解、预防和治愈癌症。“本申请中包含的所有用户都有使用IHC来解决转化研究问题的历史，这些问题使用生物标志物来表示负责介导肿瘤生长，血管生成，转移和侵袭性的关键细胞过程。申请的Dako Omnis上级PRN中使用的当前设备，原因如下：a）Dako Omnis具有温度和湿度控制，可实现前所未有的抗体染色标准化水平; B）能够同时进行原位杂交和IHC检测;）增强了为多名研究者同时轻松运行多个载玻片的能力; d）增加的载玻片容量和更短的处理时间，允许更大的通量; e）使用更少的试剂，允许在相同的成本下每次研究更多的载玻片; f）更高水平的自动化，减少错误并确保一致性。Dako Omnis以比迄今为止任何其他染色系统更可控和全面的方式解决了所有上述因素。

 DESCRIPTION (provided by applicant): Roswell Park Cancer Institute (RPCI) seeks funds for the purchase of a Dako Omnis autostainer for immunohistochemistry (IHC). The instrument will be housed in the Pathology Resource Network facility (PRN) and overseen by Dr. Angela Omilian. The PRN is supported by a Cancer Center Support Grant and has been serving researchers as a formal shared resource core at RPCI for 7 years. The histology expertise associated with the PRN facility dates to even earlier; this facility has assisted investigators wih automated IHC since 2001. Thus, PRN staff is experienced and well-suited to operate and maintain the requested equipment. In a typical year, the PRN processes at least 7500 research IHC slides and uses two autostainers on a daily basis to meet this demand. Recently, one machine has been failing and is at the end of its useful lifespan. This is the impetus for our application. IHC is a straightforward, well-established, and cost-effective platform for the detection of protein expression in human tissue. In a research setting, IHC is often used to screen tumor tissues for biomarkers that can be used for cancer diagnosis, prognosis, staging, and/or as markers of therapeutic intervention. Automated IHC via the Dako Omnis permits an unprecedented level of standardization from run to run, quality, and also allows the PRN facility to process up to 120 research slides each workday, easily serving the needs of several investigators at RPCI at once. This application includes 16 principal investigators spanning a diverse range of basic and translational research, yet all sharing the common mission of RPCI: "To Understand, Prevent, and Cure Cancer." All users included in this application have a demonstrated history of using IHC to address translational research questions using biomarkers to signify the key cellular processes that are responsible for mediating tumor growth, angiogenesis, metastasis, and invasiveness. The requested Dako Omnis is superior to the current machinery used in the PRN for the following reasons: a) the Dako Omnis has temperature and humidity controls that permit an unprecedented level of standardization for antibody staining; b) the capability to run in situ hybridization simultaneously with IHC testing; ) an enhanced ability to run multiple slides for multiple investigators simultaneously and with ease; d) an increased slide capacity and shorter processing time allowing for greater throughput; e) uses less reagents permitting more slides per study for the same cost; f) a higher level of automation that reduces errors and ensures consistency. The Dako Omnis addresses all of the aforementioned factors in a way that is more controlled and comprehensive than any other staining system to date.