Molecular Cytogenetic

项目概述（见说明）：分子细胞遗传学共享资源（MCSR）为制备适合于整个基因组的分子遗传学和细胞遗传学分析的人和鼠样品提供了工具。其目标是为癌症中心的研究人员提供在不同分辨率水平上分析基因组所需的全方位服务。这些服务包括建立eb病毒转化细胞系，从各种组织培养样本中分离DNA和mRNA，以及初级活组织检查。该设施与基因组学共享资源一起运作，为提供用于分析的基因组材料创造了一条直接管道。从中期染色体的制备到定制设计探针的生成，以及用于检测拷贝数和染色体结构改变的杂交，我们提供了全面的服务。MCSR提供所有细胞遗传学水平的分析，从标准到光谱核型，从整个染色体染色到基因组独特区域的位点特异性探针。MCSR人员经过培训，可以针对调查人员感兴趣的区域设计有针对性的、特定位点的探针。所有探头都是定制设计和内部生成的。MCSR还对石蜡包埋组织进行杂交，以及图像采集和全数据分析。

PROJECT SUMMARY (See instructions): The Molecular Cytogenetic Shared Resource (MCSR) provides tools for the preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of the entire genome. The goal is to provide Cancer Center investigators with the whole spectrum of services necessary to analyze the genome at various levels of resolution. These services include the establishment of EBV transformed cell lines and isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies. The facility operates in conjunction with the Genomics Shared Resource to create a direct pipeline for the provision of genomic material for analysis. A full panel of services is provided, from preparation of metaphase chromosomes to the generation of custom designed probes and hybridization for the detection of copy number and structural chromosomal alterations. The MCSR provides analyses at all cytogenetic levels of resolution from standard to spectral karyotyping and from whole chromosome paints to locus-specific probes for unique regions of the genome. MCSR personnel are trained to design targeted, locus-specific probes for regions of interest to investigators. All probes are custom designed and generated in-house. The MCSR also performs hybridizations on paraffin-embedded tissues as well as image acquisition and full data analysis.