Nanotechnological Regeneration of Spiral Ganglion Neurons with Human Stem Cells

描述（由申请人提供）：本提案描述了一个为期5年的干细胞生物学和纳米技术医学临床科学家职业发展培训计划。首席研究员现在是西北大学的助理教授，在那里他将通过独特的跨部门资源整合来扩展他的科学技能。这项计划将改善目前人工耳蜗技术的效果，并最终使全球2.75亿聋人恢复听力。

DESCRIPTION (provided by applicant): This proposal describes a 5 year training program for the development of a clinician-scientist career in Stem Cell Biology and Nanotechnological Medicine. The principal investigator is now an assistant professor at Northwestern University where he will expand upon his scientific skills through a unique integration of interdepartmental resources. This program will improve outcome of current technology in a cochlear implant and it would ultimately benefit the 275 million deaf people worldwide for restoring their hearing. John Kessler, M.D. will mentor the principal investigators' scientific development. Dr. Kessler is a recognized leader in the field of stem cell neurobiology. He is the director of Stem Cell Biology Institute at Northwestern University and has trained numerous postdoctoral fellows and clinician-scientists. To enhance the training, the program will enlist the expertise of Dr. Samuel Stupp, Director of Institute of BioNanotechonology in Medicine (IBNAM) at Northwestern University. Dr. Stupp pioneered the Nanotechnological application in medicine. In addition, Dr. Stefan Heller at Stanford University, who is a leading scholar in hair-cell regeneration, will also provide scientific and career advice. The proposed study will integrate our current understanding of auditory neuron regeneration. The PI will first establish a method for the controlled generation of functional human embryonic stem cell (hESCs)-derived auditory neurons using diffusible ligands and overexpression of relevant human transcription factors. Having established this goal, The PI will then develop an appropriate stem cell niche for hESC-derived auditory neurons using a combined biophysical and biochemical approach involving nanotechnology and neurotrophic factors. The PI has the great fortune to be situated at a unique institute at Northwestern University, where both a human Pluripotent Stem Cell (hPSC) core facility and one of the largest bionanotechnology communities in the world co- exist in one academic institute. It is access to this technology and the support of PI's mentors, which makes this proposed work feasible. Department of Otolaryngology-Head and Neck Surgery Northwestern University provides an ideal setting for training clinician-scientists by incorporating expertise from diverse resources into customized programs. Such an environment maximizes the potential for the principal investigator to establish a scientific niche from which an academic career can be constructed.