Molecular Cell and Analyitical Services Facility Core

分子细胞和分析服务设施核心(MCASFC)的总体目标是提供 中心成员接受教育，并可以使用最先进的工具来评估 环境对人类和哺乳动物基因组和表观基因组的影响。核心还提供分析 服务，特别是暴露生物的细胞和组织中的金属分析服务，因为金属效应 基因组和表观基因组是该中心的专业研究优势。的基本原理，以及 这一核心的不断演变反映了环境健康研究的当前趋势，特别是 快速变化的基因x环境互作领域。在功能上很重要，与剂量相关的扰动 环境应激源(如金属、致癌物、空气污染颗粒物)对人类健康的影响 由研究基因表达变化的中心研究人员积极分析，表观遗传调控 候选基因和全基因组水平、细胞周期扰动、蛋白质表达和信号通路 互动。MCASFC支持中心研究人员使用基因组学和表观基因组学资助的研究 确定对健康的毒性挑战的生物标志物和预测性哨兵，以及确定目标 预防和治疗干预措施。2008年实施并由该核心监督的一项新倡议是 协作性酵母筛选项目包括分析金属诱导的全球基因表达变化 和臭氧。这个新项目部分由中心主任的基金支持，涉及多个中心 纽约州奥尔巴尼基因中心的托马斯·贝格利博士的调查人员。它已经变得越来越多 显然，表观基因组在调节生物体对 暴露在环境中。因此，自上次续订以来为该核心制定的其他新计划 ChlP-Chip、ChlP-Seq和RNA-Seq的掺入是表观遗传和转录组生物标记物 发现并调查对环境因素的毒性和疾病反应的潜在机制。 基因组/表观基因组x环境相互作用的研究需要多学科的方法和 在最先进的仪器和新兴技术的支持下利用高灵敏的分子技术 技术。MCASFC为中心调查人员提供了对专门方法的不断发展的访问 和设备，通过利用涉及大学内部和大学间的区域资源，现场或非现场 研究伙伴关系。MCASFC在认为必要时，通过继续 利用竞争性和机构资金来源的资金。此外，由于基因组/表观基因组 在实施之前，研究需要大量的培训，设施核心提供中心调查人员 就最新技术的使用、应用、限制和挑战提供咨询服务。

The overall objective of the Molecular Cell and Analytical Services Facility Core (MCASFC) is to provide Center members with education on, and access to, state-of-the-art tools for assessing the effects of the environment on human and mammalian genomes and epigenomes. The Core also provides analytical services, particularly those for metals analysis in cells and tissues of exposed organisms, since metal effects on the genome and epigenome are specialized research strengths of the Center. The rationale for, and continuing evolution of, this Core reflects current trends in environmental health research, and in particular the rapidly changing field of gene x environment interactions. Functionally important, dose-related perturbations to human health from environmental stressors (e.g. metals, carcinogens, air pollution particulates) are being actively analyzed by Center researchers who study changes in gene expression, epigenetic regulation at candidate gene and whole genome levels, cell cycle perturbations, protein expression and signaling pathway interactions. The MCASFC supports Center investigators' funded studies using genomics and epigenomics to identify biomarkers and predictive sentinels of toxic challenges to health, as well as to identify targets for preventive and therapeutic interventions. One new initiative implemented in 2008 and overseen by this core is the collaborative yeast screen project involving analysis of global gene expression changes induced by metals and ozone. This new project, supported in part by the Center Director's fund, involved multiple Center investigators with Dr. Thomas Begley at the GeNYsis Center in Albany, NY. It has become increasingly evident that the epigenome plays an enormous role in modulating the responses of organisms to environmental exposures. Thus, other new initiatives that have developed for this Core since the last renewal are the incorporation of ChlP-Chip, ChlP-Seq and RNA-Seq for epigenetic and transcriptome biomarker discovery and for investigating mechanisms underlying toxic and disease responses to environmental agents. Research on genome/epigenome x environment interactions requires multidisciplinary approaches and utilization of highly sensitive molecular techniques supported by state-of-the-art instruments and emerging technologies. The MCASFC provides Center investigators with evolving access to specialized methodologies and equipment, either on-site or off-site, by taking advantage of regional resources involving intra- and interuniversity research partnerships. The MCASFC acquires new equipment as deemed necessary, by continuing to leverage funds from competitive and institutional funding sources. Furthermore, since genome/epigenome studies require considerable training prior to implementation, the Facility Core provides Center investigators with consultation services on the uses, applications, limitations and challenges ofthe latest techniques.