Genetic Mouse Models

小鼠遗传模型已被CCBMB研究人员广泛使用，并在 肌肉骨骼生物学和医学。动物模型核心旨在促进最先进的研究 通过支持新的优化和实施为CCMBM研究人员提供环境 技术，将在CCBMB研究人员中广泛使用的小鼠遗传工具的产生， 及时可靠地生产、保存和分享转基因小鼠，以及 探索斑马鱼作为肌肉骨骼研究的模型。加强鼠标的有效使用 和斑马鱼模型生物在肌肉骨骼生物学方面的研究，动物模型核心将 促进实施TALEN和CRISPR/CAS基因组编辑技术。这项技术将 通过促进研究人员发起的小鼠和斑马鱼动物的发育，使CCBMB研究人员受益 模型，并通过开发针对Cre重组酶、LacZ和rosa26基因座的“通用”TALEN， 以允许对特征良好的小鼠等位基因进行有效的重新设计。建立这种基因组编辑 技术将使CCBMB成员受益，因为它能够快速、经济地生产新鼠标和 斑马鱼模型，并允许对具有良好特征的现有小鼠品系进行基因改造， 有明确的时间和空间表达模式。动物模型核心关注点的其他目标 关于咨询和教育以及维护一个全面的数据库以促进动物共享 模型和遗传工具。

Mouse genetic models have been extensively utilized by CCBMB investigators and are central to research in musculoskeletal biology and medicine. The Animal Models Core aims to foster a state-of-the-art research environment for CCMBM researchers by supporting the optimization and implementation of new technologies, the generation of mouse genetic tools that will have wide use among CCBMB researchers, the production, preservation, and sharing of genetically altered mice in a timely and reliable manner, and the exploration of the zebrafish as a model for musculoskeletal research. To enhance the effective use of mouse and zebrafish model organisms for research in musculoskeletal biology, the Animal Models Core will facilitate the implementation of TALEN and CRISPR/Cas genomic editing technology. This technology will benefit CCBMB investigators by facilitating investigator-initiated development of mouse and zebrafish animal models, and by developing "universal" TALENs for targeting Cre recombinase, LacZ, and the ROSA26 locus, to allow efficient re-engineering of well-characterized mouse alleles. Establishing this genomic editing technology will benefit CCBMB members by enabling fast and cost-effective production of new mouse and zebrafish models and by allowing the genetic modification of well-characterized existing mouse lines that have well-defined temporal and spatial expression patterns. Additional aims of the Animal Models Core focus on consultation and education and the maintenance of a comprehensive database to foster sharing of animal models and genetic tools.