Nutrient Interaction in Prostate Cancer Cells: Effects of I3C/DIM on AhR-mediated pathway

该协议的目的是确认I3 C/DIM对动物中前列腺癌相关免疫细胞行为的影响的体外研究结果，为细胞反应提供生理环境。研究设计涉及使用各种分子生物学技术（包括DNA深度测序和转录组分析）表征机制。 具体而言，本研究将阐明I3 C/DIM对体内肿瘤相关免疫细胞（包括巨噬细胞和Th 17细胞）影响的分子机制，并检查I3 C/DIM的癌症预防作用是否与免疫细胞介导的信号通路的变化相关。

The goal of this agreement is to confirm in vitro findings of I3C/DIM effects on prostate cancer-associated immune cell behavior in animals that provide physiological environment for cellular response. The study design involves characterization of the mechanism using a variety of molecular biology techniques including DNA deep sequencing and transcriptome analyses. Specifically, this study will elucidate the molecular mechanism explaining the influence of I3C/DIM on tumor-associated immune cells including macrophages and Th17 cells in vivo and examine if the cancer preventive effects of I3C/DIM are associated with the changes in immune-cell mediated signaling pathways.