Mini Chromosome and Fluorescent Proteins for Expression of Protein Complexes

总结/摘要 由人类基因组编码的蛋白质可能参与多达20万对蛋白质 “几乎每个细胞中的主要过程都是由10个或更多的组件进行的， 互锁蛋白质分子”（布鲁斯艾伯茨，细胞1998）。然而，没有市售的 用于调节多亚基蛋白复合物表达的表达系统。现在迫切需要 对于可以产生多蛋白复合物的哺乳动物表达系统，受到严格调控， 在长期培养中稳定，并且不整合。没有报告的表达系统接近会议 所有这些需求。Lucigen将开发和商业化一种稳定的附加型表达系统， 无偏克隆能力，可调光诱导启动子，改进的荧光标记，以及 表达等量的多种蛋白质。该系统将便于直接实验 通过计算模型预测的无数蛋白质复合物的验证，以及那些 已知的（例如，离子通道、多亚基酶、生物化学途径）。

Summary/abstract The proteins encoded by the human genome may participate in as many as 200,000 pairwise protein interactions, and “nearly every major process in a cell is carried out by assemblies of 10 or more interlocking protein molecules” (Bruce Alberts, Cell 1998). However, there are no commercially available expression systems for regulated expression of multi-subunit protein complexes. There is an acute need for a mammalian expression system that can produce multi-protein complexes, is tightly regulated, stable in prolonged culture, and non-integrated. No reported expression system comes near to meeting all these needs. Lucigen will develop and commercialize a stable episomal expression system with unbiased cloning ability, tunable light-inducible promoters, improved fluorescent tags, and the ability to express equivalent amounts of multiple proteins. This system will facilitate direct experimental validation of the myriad protein complexes predicted by computational models, as well as those that are already known (e.g, ion channels, multi-subunit enzymes, biochemical pathways).