ShEEP Request for Flow Cytometer Special Order Laser Upgrade

摘要 在此应用程序中，我们要求对我们现有的Becton Dickinson LSRII流式细胞仪进行以下升级： 1)安装相干G4355 nm 60 mW紫外激光器 2)安装在八角形光学阵列上的三个光电倍增管和配置的光学元件： A.BUV396(379/28) B.BUV496(450LP；515/30) C.BUV737(690LP；740/35) 3)在现有的带有BV785光学元件的紫色八角形上增加额外的光电倍增管(750LP；780/60) 4)支持新配置的额外电子设备 5)FACS流量供给系统 这些升级将把我们可以分析的总颜色数量增加到18种，并增强LSRII的 性能容量。以下研究项目将得到升级系统的支持： 1)Marc Goalstone，博士，首席研究员：Blr&D功绩回顾奖《糖尿病：ERK5和 血管炎症“ 2)Robert Keith，医学博士，首席研究员：Blr&D功绩评审奖：肺的PPAR伽马激动剂 癌症的化学预防“ 3)Elizabeth Redente，博士，首席研究员：Blr&D职业发展奖 肿瘤坏死因子-α介导的肺纤维化消退 4)Subbiah Pugazhenthi博士，首席研究员：BR&D功绩评审奖《CREB的机制》 阿尔茨海默病的大脑调节失调“ 5)Jane Reusch，医学博士，首席研究员：Blr&D功绩评审奖 糖尿病血管功能障碍“ 6)丽莎·布伦纳(Lisa Brenner)，博士，CSR&D功绩评审奖联合首席研究员 犬尿氨酸途径与退伍军人的自杀行为 7)Ehrin Armstrong，医学博士，本地站点调查员：CSR&D功绩评审奖 强化排脂的祖细胞动员

Abstract In this application we request the following upgrades to our existing Becton Dickinson LSRII Flow Cytometer: 1) Installation of a Coherent G4 355nm 60mw UV laser 2) Three photomultiplier tubes installed on an Octagon optical array and optics configured: a. BUV396 (379/28) b. BUV496 (450LP;515/30) c. BUV737 (690LP; 740/35) 3) Additional photomultiplier tube added to existing violet Octagon with optics for BV785 (750LP; 780/60) 4) Additional electronics to support the new configuration 5) FACS Flow Supply System These upgrades will increase the number of total colors we can analyze to 18 and enhance the LSRII’s performance capacity. The following research projects will be supported by the upgrades system: 1) Marc Goalstone, PhD, Principal Investigator: BLR&D Merit Review Award “Diabetes: ERK5 and Vascular Inflammation” 2) Robert Keith, MD, Principal Investigator: BLR&D Merit Review Award “PPAR gamma Agonists for Lung Cancer Chemoprevention” 3) Elizabeth Redente, PhD, Principal Investigator: BLR&D Career Development Award “Mechanisms of TNF-alpha Mediated Resolution of Pulmonary Fibrosis” 4) Subbiah Pugazhenthi, PhD, Principal Investigator: BLR&D Merit Review Award “Mechanism of CREB dysregulation in Alzheimer brain” 5) Jane Reusch, MD, Principal Investigator: BLR&D Merit Review Award “Pharmacological Restoration of Diabetic Vascular Dysfunction” 6) Lisa Brenner, PhD, Co-Principal Investigator: CSR&D Merit Review Award “Toxoplasma gondi, the kynurenine pathway, and suicidal behavior in veterans” 7) Ehrin Armstrong, MD, Local Site Investigator: CSR&D Merit Review Award “Plaque Regression and Progenitor Cell Mobilization with Intensive Lipid Elimination”