ShEEP Request for Imaging Flow Cytometer System

Amnis Image Stream mark II流式细胞仪与退伍军人健康直接相关 通过结合联合收割机的速度、灵敏度和表型分析能力的能力， 流式细胞术与显微镜的详细图像和功能见解。双 流式细胞仪的能力将大大提高数据应用（细胞-细胞研究 相互作用，吞噬作用，凋亡和自噬，循环肿瘤的表征 细胞等）退伍军人事务部资助的调查员戈尔丁博士研究人类的作用 风湿病退伍军人中的调节性T细胞达维拉博士研究了先天 免疫Toll样受体（TLR）在与免疫治疗相关的适应性T细胞应答中的作用 癌Tonelli博士专注于炎症过程对大脑功能的影响， 行为及其对心理健康和压力相关疾病的影响。普赖尔医生 研究运动训练改善治疗和预防的机制 2型糖尿病富尔顿博士研究减少乳腺癌的考克斯-2途径 Mortality. Keegan博士研究哮喘和过敏的机制。Gartenhaus博士 研究了弥漫性大B细胞淋巴瘤发病率增加的机制基础， 淋巴瘤（DLBCL）与衰老相关。贝尔博士研究新的方法来改善 血液恶性肿瘤的化学治疗。罗斯博士研究小说的作用 补体受体在炎症和自身免疫中的作用Donahue博士研究细胞凋亡 和细胞周期分析。 为巴尔的摩VA购置AMNIS Image Stream mark II流式细胞仪系统 核心研究实验室将大大加强目前正在进行的项目 通过VA调查员，也有助于促进新的和创新的项目。该系统是一 能够捕获个体的实时图像的最先进的成像流式细胞仪 细胞与其他任何流式细胞仪不同，AMNIS图像流不仅记录了 在每个细胞的基础上细胞蛋白质;而且还提供了亚细胞的直接信息 本地化这允许直接测量影响所有细胞的关键细胞信号传导事件。 细胞生物学方面包括：癌症，免疫学，代谢，神经学，衰老， 血液学目前退伍军人事务部调查人员的项目依赖于结合旧的，效率较低的 技术现在可以通过这一个系统做出新的发现。

The Amnis Image Stream mark II Flow Cytometer has direct relevance to Veteran Health Care as exemplified by the ability to combine speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. The dual capability flow cytometer will greatly enhance the data applications (study of cell-cell interactions, phagocytosis, apoptosis and autophagy, the characterization of circulating tumor cells, etc.) of a number of VA funded investigators. Dr. Golding studies the role of human regulatory T cells in Veterans with rheumatologic disease. Dr. Davila studies the role of innate immune Toll-like receptors (TLR) in adaptive T-cell responses relevant to immunotherapy for cancer. Dr. Tonelli focuses on the effects of inflammatory processes on brain function and behavior and their implications for mental health and stress related disorders. Dr. Prior examines the mechanisms by which exercise training improves the treatment and prevention of type 2 diabetes. Dr. Fulton investigates the COX-2 Pathway to Reduce Breast Cancer Mortality. Dr. Keegan investigates mechanisms of asthma and allergy. Dr. Gartenhaus investigates the mechanistic basis for the increased incidence of diffuse large B-cell lymphoma (DLBCL) associated with aging. Dr. Baer studies new ways to improve chemotherapeutic treatment of hematologic malignanacies. Dr. Rus studies the role of novel complement receptors in inflammation and autoimmunity. Dr. Donahue investigates apoptosis and cell cycle analysis in esophageal cancer cells. The acquisition of an AMNIS Image Stream mark II Flow Cytometer system for the Baltimore VA Research Laboratory Core would significantly enhance the projects currently being carried out by VA investigators, and also help to promote new and innovative projects. This system is a state-of-the art imaging flow cytometer that is capable of capturing real-time images of individual cells. Unlike any other flow cytometer, the AMNIS Image Stream not only records the presence of cellular proteins on a per-cell basis; but also provides direct information on sub-cellular localization. This allows for direct measurement of critical cell signaling events that impact all aspects of cell biology including: cancer, immunology, metabolism, neurology, senescence, and hematology. Current VA investigators’ projects that rely on combining older, less efficient technologies would now be able to make new discoveries with just this one system.