Ribosomally Synthesized Proteins Incorporating Modified Dipeptides

项目摘要 这项研究的长期目标涉及修饰肽基转移酶（PTC）中心， 细菌核糖体，使非常不寻常的氨基酸不能识别的野生型， 型核糖体。近年来，我们描述了修饰核糖体的选择和它们的用途 在将D-氨基酸、β-氨基酸、二肽和二肽模拟盒掺入 proteins.在拟议的五年研究期间，工作重点将是引进 构象受限的环状二肽和核碱基氨基酸转化为特定的蛋白质 (both如单肽和二肽），创造具有新特性的物种， 分析要实现的蛋白质功能，以及分析特定的构象特性 对酶功能和增强（和潜在修饰）蛋白质DNA的策略至关重要 识别.

Project Summary The long term goals of this research involve modification of the peptidyltransferase (PTC) center in bacterial ribosomes to enable the incorporation of very unusual amino acids not recognized by wild- type ribosomes. In recent years, we have described the selection of modified ribosomes and their use in incorporating D-amino acids, beta amino acids, dipeptides and dipeptidomimetric cassettes into proteins. During the five years of proposed research, the focus of efforts will be on the introduction of conformationally constrained cyclic dipeptides and nucleobase amino acids into specific proteins (both as mono- and dipeptides), creating species that have novel properties enabling enhanced analysis of protein function to be realized, as well as the analysis of specific conformational properties essential for enzyme function and strategies for enhancing (and potentially modifying) protein‒DNA recognition.