Molecular Cytogenetic

项目摘要(见说明)：分子细胞遗传学共享资源(MCSR)为制备适合于整个基因组的分子遗传学和细胞遗传学分析的人类和小鼠样本提供了工具。其目标是为癌症中心的研究人员提供在不同分辨率水平上分析基因组所需的全套服务。这些服务包括建立EBV转化的细胞系，从各种组织培养样本和初级活检组织中分离DNA和mRNA。该设施与基因组学共享资源一起运作，为提供用于分析的基因组材料创建了一个直接管道。提供一整套服务，从中期染色体的准备到定制设计的探针的生成和用于检测拷贝数和结构染色体变化的杂交。MCSR提供所有细胞遗传学水平的分析，从标准核型分析到光谱核型分析，从整个染色体涂色到基因组独特区域的特定位点探针。MCSR人员接受培训，为调查人员感兴趣的区域设计有针对性的、特定于位置的探针。所有探头都是内部定制设计和生成的。MCSR还对石蜡包埋的组织进行杂交，以及图像采集和完整的数据分析。

PROJECT SUMMARY (See instructions): The Molecular Cytogenetic Shared Resource (MCSR) provides tools for the preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of the entire genome. The goal is to provide Cancer Center investigators with the whole spectrum of services necessary to analyze the genome at various levels of resolution. These services include the establishment of EBV transformed cell lines and isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies. The facility operates in conjunction with the Genomics Shared Resource to create a direct pipeline for the provision of genomic material for analysis. A full panel of services is provided, from preparation of metaphase chromosomes to the generation of custom designed probes and hybridization for the detection of copy number and structural chromosomal alterations. The MCSR provides analyses at all cytogenetic levels of resolution from standard to spectral karyotyping and from whole chromosome paints to locus-specific probes for unique regions of the genome. MCSR personnel are trained to design targeted, locus-specific probes for regions of interest to investigators. All probes are custom designed and generated in-house. The MCSR also performs hybridizations on paraffin-embedded tissues as well as image acquisition and full data analysis.