Humanized Mouse Core

核心A项目总结/摘要 该核心的主要目标是产生人源化小鼠，其可用于测试HCMV潜伏期， 再活化以及使用项目1-5中产生的HCMV突变体的造血。的长期目标 核心A是产生huBLT小鼠以回答HCMV潜伏期、再活化和再活化的体内问题。 基于该计划中各种研究中提出的体外CMV模型的造血 项目首先，核心将产生huBLT小鼠，以分析项目1-4中产生的HCMV突变体， 在用G-CSF和AMD-3100治疗后建立和维持潜伏期并重新激活的能力。一旦 项目1-4将确定对HCMV重要的信号通路和/或分泌因子 潜伏期和重新激活，核心A将使用化学抑制剂，中和抗体， 表达shRNA的重组HCMV和/或添加再活化所需的细胞因子/生长因子。 其次，核心将分析感染WT HCMV（项目5）或病毒的huBLT小鼠中的造血 突变体（项目1-4），然后将使用化学抑制剂，中和抗体， 表达shRNA的重组HCMV和/或添加造血所需的细胞因子/生长因子。

CORE A PROJECT SUMMARY/ABSTRACT The primary goal of this Core is to generate humanized mice that can be used to test HCMV latency and reactivation as well as hematopoiesis using HCMV mutants generated in Projects 1-5. The long-term goal of Core A is to generate huBLT mice to answer in vivo questions of HCMV latency, reactivation and hematopoiesis based on in vitro CMV models proposed in the various studies embedded in the Program Project. First, the Core will generate huBLT mice to analyze HCMV mutants generated in Projects 1-4 for their ability to establish and maintain latency and reactivate following treatment with G-CSF and AMD-3100. Once Projects 1-4 will have identified signaling pathways and/or secreted factors that are important for HCMV latency and reactivation, Core A will validate them using chemical inhibitors, neutralizing antibodies, recombinant HCMV expressing shRNAs and/or addition of cytokines/growth factors required for reactivation. Second, the Core will analyze hematopoiesis in huBLT mice infected with WT HCMV (Project 5) or viral mutants (Projects 1-4) and then will validate the results using chemical inhibitors, neutralizing antibodies, recombinant HCMV expressing shRNAs and/or addition of cytokines/growth factors required for hematopoiesis.