Single chemosensory cells in the salivary gland.

摘要 唾液腺导管充当腺体的分泌组分与口腔粘膜之间的上皮屏障。 腔这种屏障的基本功能是检测和响应外部侵害。在许多组织中， 分散的单个化学感受细胞（SCC）起着哨兵的作用，将外部线索传递到转导的细胞中。 激活免疫反应的信号。在小鼠和人类的唾液腺中，我们发现了不同的 孤管细胞共表达化学感受信号通路的组分，包括Nkcc 1，IP3R3， 和Plcß2。在小鼠中，这些细胞通过转录因子Ascl3的表达来标记。的 观察到这些形态上不同的细胞表现出类似SCC的分子特征， 表明它们有特殊的功能这项提议将探讨我们的假设，即SCC是活跃的 在小鼠和人的唾液腺中，并参与检测和转导外部刺激， 周围的细胞，也许还有神经元。我们推测Ascl3表达标志着SCC的一种类型， 小鼠唾液腺，并将研究这是否也是人类腺体的情况。我们预计 SCC在唾液腺中产生免疫应答中起关键作用，并可能参与免疫应答的调节。 导致急性和慢性炎症的机制。

Abstract The salivary gland ducts serve as an epithelial barrier between secretory components of the gland and the oral cavity. An essential function of such a barrier is the detection and response to external insults. In many tissues, scattered single chemosensory cells (SCCs) function as sentinels, which relay external cues into transduced signals that activate an immune response. In both mouse and human salivary glands, we have found distinct solitary duct cells that co-express components of chemosensory signaling pathways, including Nkcc1, IP3R3, and Plcß2. In the mouse, these cells are marked by expression of the transcription factor Ascl3. The observation that these morphologically distinct cells exhibit molecular characteristics resembling SCCs suggests that they fulfill a specialized function. This proposal will explore our hypothesis that SCCs are active in mouse and human salivary glands, and are involved in detection and transduction of external stimuli to surrounding cells, and perhaps to neurons. We speculate that Ascl3 expression marks a type of SCCs in the mouse salivary gland, and will investigate whether this is the case in human glands as well. We expect that SCCs play a critical role in mounting an immune response in the salivary glands, and may be involved in the mechanisms leading to acute and chronic inflammation.