Experimental Pathology Laboratory Shared Resource

项目总结/摘要 实验病理学实验室（EPL）的使命是提供一系列全面的 组织病理学服务和专业知识，使UWCCC成员能够充分利用动物的力量 人类癌症模型，以揭示有助于癌症发展，进展和 对治疗的反应。EPL的工作人员在组织学、组织病理学和癌症方面拥有丰富的专业知识 生物学和共同拥有近100年的癌症研究经验。EPL总共服务了99个 UWCCC成员在当前CCSG资助周期内，每年有43至58名独立成员 在此期间使用EPL服务。使用EPL的会员数量和服务量 自CCSG上次审查以来，提供的服务有所增加，2016年成员调查的数据表明， 使用将继续增加。EPL优先考虑以下三个目标：目标1是提供基本的组织学 为UWCCC成员提供服务。我们的成员利用各种各样的动物模型来解决重要的 有关癌症病因学、预防和治疗的问题。EPL支持这项研究，提供 必要的组织学服务和专业知识。UWCCC研究人员收集的组织标本是 运输到EPL，在那里根据 研究者的要求和我们的标准化操作规程。EPL提供普通染色 我们的染色方案，例如苏木精和伊红（H&E），几乎可以执行任何特殊染色。专家 病理咨询是由博士露丝沙利文，一个委员会认证的兽医病理学家和EPL的工作人员提供 会员是否目的2是促进正常和肿瘤组织的分子分析。UWCCC成员 经常评估作为生物标志物或治疗剂的特定蛋白质的表达、功能或定位， 目标的根据成员的要求，EPL将开发和实施定制的免疫组织化学 (IHC)或免疫荧光（IF）方案。我们的成员认为， 服务是无价的，已经开发、优化和验证了70多种独特的IHC或IF方案 在当前的CCSG资助周期内，EPL。目的3是为UWCCC提供组织病理学专业知识 成员和研究人员。EPL的工作人员可随时咨询样品制备 和操作或操作我们的尼康荧光显微镜进行图像采集。沙利文博士提供 每月的幻灯片评论和教程，以UWCCC研究人员免费。另外，沙利文医生 做一般病理咨询CCSG的支持使我们能够优先为UWCCC成员提供 以低于商业实验室的费用获得高质量的组织病理学服务， 免费向成员提供专家咨询支持。

PROJECT SUMMARY / ABSTRACT The mission of the Experimental Pathology Laboratory (EPL) is to provide a comprehensive array of histopathology services and expertise that enable UWCCC members to fully exploit the power of animal models of human cancer to reveal mechanisms that contribute to cancer development, progression, and responsiveness to therapies. EPL staff have extensive expertise in histology, histopathology, and cancer biology and together possess nearly 100 years of experience in cancer research. EPL has served a total of 99 UWCCC members during the current CCSG funding cycle, and between 43 and 58 unique members per year used EPL services during this interval. Both the number of members using EPL and the volume of services provided have increased since the CCSG was last reviewed and data from a 2016 survey of members indicate use will continue to increase. EPL prioritizes the following three aims: Aim 1 is to provide essential histology services to UWCCC members. Our members utilize a wide variety of animal models to address important questions pertaining to cancer etiology, prevention, and therapy. The EPL supports this research by providing essential histology services and expertise. Tissue specimens collected by UWCCC investigators are transported to the EPL where they are processed, embedded, sectioned, and stained according to the requirements of the investigator and our Standardized Operating Procedures. EPL offers common staining protocols, for example hematoxylin and eosin (H&E), and can perform virtually any special stain. Expert pathology consultation is provided by Dr. Ruth Sullivan, a board certified veterinary pathologist and EPL staff member. Aim 2 is to facilitate the molecular analyses of normal and neoplastic tissues. UWCCC members frequently evaluate expression, function, or localization of specific proteins as biomarkers or therapeutic targets. Upon request of a member, the EPL will develop and implement a custom immunohistochemistry (IHC) or immunofluorescence (IF) protocol that is specific to a protein of interest. Our members view this service as invaluable and over 70 unique IHC or IF protocols have been developed, optimized, and validated by EPL during the current CCSG funding cycle. Aim 3 is to provide histopathology expertise to UWCCC members and researchers. EPL staff members are available for walk-in consultation on sample preparation and handling or on operation of our Nikon fluorescence microscope for image acquisition. Dr. Sullivan provides monthly slide reviews and tutorials to UWCCC researchers free of charge. In addition, Dr. Sullivan is available for general pathology consults. Support from the CCSG allows us to provide UWCCC members with priority access to high quality histopathology services at costs below those of commercial laboratories as well as to provide expert consultative support at no cost to members.