Waters ACQUITY UPLC M-Class with NanoLockSpray ionization source

摘要 目前的设备补充请求是为了升级现有的质谱学 使纳米级高效液相色谱成为可能的平台。现役补助金R01 GM099989(PI：LEE)， 和R01的GM130810和GM121603(PI：Atkins)共同申请沃特斯M级的资金 用相应的NanoLockSpray电离源进行高效液相色谱分析。此次升级将为您提供 提高灵敏度，以克服灵敏度和材料要求方面的限制 阻碍了对许多感兴趣的蛋白质的分析表征。这将是 极大地增加了我们可以分析的系统和样本的范围。这些设备将是 在由NIGMS资助的三个PI之间平均分配，同时由 药学院的质谱学设施。

ABSTRACT The current equipment supplement request is for an upgrade of the current mass spectrometry platform to enable nano-scale liquid chromatography. Active grants R01 GM099989 (PI: Lee), and R01s GM130810 and GM121603 (PI: Atkins) jointly request the funds for a Waters M-class HPLC with the corresponding NanoLockSpray ionization source. The upgrade will provide the enhancement in sensitivity to overcome the limits in sensitivity and material requirements that have hindered analytical characterization of numerous the proteins of interest. This will dramatically increase the range of systems and samples we can analyze. The equipment will be shared equally among three NIGMS-funded PIs while being managed and maintained by the School of Pharmacy Mass Spectrometry Facility.