Control of carbohydrate utilization in the prominent gut bacterium Bacteroides thetaiotaomicron

项目总结 这项建议通过研究类杆菌来研究微生物对营养物质的反应。 Taotaomicron是一种重要的肠道共生细菌，可以在大量 人类和许多其他微生物无法利用的碳水化合物。~18%的B. 据预测，taotaomicron的基因组将介导碳水化合物的吸收和分解，而 对这些过程的监管。这项提议试图确定控制四个 碳水化合物利用的调节剂，以定义它们的调节目标，并确定这些 蛋白质发挥其调节作用。首先，我们将调查一位大师的控制和控制 碳水化合物利用调节与几种类杆菌的肠道定植有关。 这一目标还探索了伸长系数EF-G的平行曲线的作用，该平行曲线受 主调节器，和主调节器一样，对于肠道定植和 碳水化合物利用率。第二，我们将研究葡萄糖是人类最喜欢的碳源 大多数生物沉默肠道定植所必需的转录激活物的表达 以一种依赖饮食的方式。第三，我们将确定基本转录如何 终止因子Rho，它在B。 它采用不同的形式，控制肠道所需的不同基因集 定居和碳水化合物利用。我们的研究将揭示这一机制和 控制碳水化合物利用的等级，并阐明了两个类Pron的作用 细菌基因调控领域。拟议的调查可能会被证明是例证。 因为他们关注的是非典型调控蛋白。此外，它们可能有助于解释 使B.thetaiotaomicron成为一个成功的肠道定殖者，并使其在瘦肉健康中的丰富 个人。

PROJECT SUMMARY This proposal examines the microbial responses to nutrients by investigating Bacteroides thetaiotaomicron, a prominent gut commensal bacterium that can grow on a large number of carbohydrates that humans and many other microbes cannot utilize. ~18% of the B. thetaiotaomicron's genome is predicted to mediate carbohydrate uptake and breakdown, and the regulation of these processes. This proposal seeks to identify the signals controlling four regulators of carbohydrate utilization, to define their regulated targets, and to establish how these proteins exert their regulatory actions. First, we will investigate the control of and by a master regulator of carbohydrate utilization implicated in gut colonization in several Bacteroides species. This aim also explores the role of a paralog of elongation factor EF-G that is under control of the master regulator, and like the master regulator, it is necessary for gut colonization and carbohydrate utilization. Second, we will examine how glucose, the preferred carbon source in most organisms, silences expression of a transcriptional activator necessary for gut colonization in a diet-dependent manner. And third, we will determine how the essential transcription termination factor Rho, which harbors a prion-like domain-containing element in B. thetaiotaomicron, adopts different forms and controls different sets of genes required for gut colonization and carbohydrate utilization. Our studies will reveal the mechanisms and hierarchies governing carbohydrate utilization, and elucidate the role of both of a prion-like domain in bacterial gene regulation. The proposed investigations may prove paradigmatic because they focus on atypical regulatory proteins. In addition, they may help explain what makes B. thetaiotaomicron a successful gut colonizer, and its abundance in lean healthy individuals.