Cell Culture and iPS Core

项目总结(细胞培养和iPS核心) 细胞培养和iPS核心(CCIC)设施在宾夕法尼亚大学校园和 区域。它满足了消化和肝病分子研究中心的巨大需求 (简称CMSDLD，是NIDDK P30消化系统疾病研究核心中心成员) 与原代细胞和已建立的细胞系相关的特定目标潜在的服务和技术，3D 培养系统(器官类型培养和类器官培养)，通过RNA控制细胞系中的基因表达 干扰和CRISPR/Cas9，以及已被诱导的多能干细胞(IPS)的应用 从基因上重新编程到胚胎干细胞样的状态，并将这种细胞分化为离散的 谱系(胃肠道、胰腺、肝脏)。此外，CCIC提供了丰富的c细胞系(2D和3D)存储库， 对身份、通过和没有支原体感染进行了良好的注释，从而提供了质量控制， 严密性和可重复性。CCIC提供标准化的协议和定期的指导和指导， 事实证明，这些都是具有成本效益的措施。有一些与使用iPS技术相关的新兴项目 纠正肠道和肝细胞的疾病状态，为转化医学提供显著的例证 CCIC。通过其服务、技术、质量控制和时间/成本效益，CCIC不断进步 CMSDLD代表成员/准成员/人员的愿景和使命。

PROJECT SUMMARY (CELL CULTURE AND iPS CORE) The Cell Culture and iPS Core (CCiC) facility is unique on the University of Pennsylvania campus and in the region. It serves a tremendous need by Center of Molecular Studies in Digestive and Liver Diseases (abbreviated as CMSDLD, which is the NIDDK P30 Digestive Diseases Research Core Center) members for services and technologies underlying Specific Aims related to primary cells and established cell lines, 3D culture systems (organotypic culture and organoids), manipulating gene expression in cell lines through RNA interference and CRISPR/Cas9, and the application of induced pluripotent stem cells (iPS) that have been genetically reprogrammed to an embryonic stem cell-like condition and differentiating such cells to discrete lineages (GI, pancreas, liver). Furthermore, the CCiC provides a rich repository of c cell lines (2D and 3D) that are well annotated for identity, passage and free of Mycoplasma infection thereby providing quality control, rigor and reproducibility. The CCiC provides standardized protocols and regular orientation and instruction, which prove to be cost-effective measures. There are emerging projects related to the use of iPS technology to correct disease states in enteroids and hepatocytes, remarkable illustrations of translational medicine fueled by the CCiC. Through its services, technologies, quality control and time/cost-effectiveness, the CCiC advances the CMSDLD's vision and missions on behalf of members/associate members/personnel.