ICAL: Impact of Cannabinoids Across Lifespan: Animal Core

项目总结：动物核心 动物核心的主要目标是提供标准化、一致的青少年和 用四氢大麻酚或其载体处理的成年小鼠和大鼠，供国际化学品管理研究所所有项目使用。的 核心的训练有素的工作人员将安排治疗，行为测试和组织收集，并将 给不同年龄、治疗方案和剂量的动物施用THC。治疗后和 洗脱后，动物将被转移到各个ICAL项目的实验室（分子，突触，和 行为）。动物核心的第二个目标是促进一致的和经过充分验证的 化学遗传学（DREADD）电路控制方法在ICAL项目。核心将提供空间 和用于病毒载体注射的仪器（立体定位框架、病毒注射设备），并且将 对实验室工作人员进行程序和方案培训，以确保一致实施。芯会 还维持和分配转基因Cre驱动小鼠和大鼠品系的集落， 靶向神经元亚型的化学遗传学操作，包括多巴胺（TH：Cre或DAT：Cre）， 如个别项目所述。动物核心的第三个目标是建立和维持 从青春期接受治疗的动物中获得的生物材料的注释储存库，或 成年时使用THC或车辆。来自各种ICAL的生物材料（脑外周组织、粪便） 活动（药代动力学和药效学研究、表型研究、项目）将 收集后用计算机化条形码系统编目，并储存在-80 ℃。一份附加说明 将创建描述可用样本和相关行为/生理数据的数据库 并应要求提供给全世界合格的研究人员。这种独特的资源，第一次在 它的类型，将（i）促进ICAL和世界各地的不同研究小组之间的新的合作; 以及（ii）开放青少年大麻素研究，让那些没有能力进行类似研究的群体进行研究。 治疗方案本身。总之，动物核心将是ICAL成功的关键， 更广泛的影响。核心将开发和实施充分表征的、一致的、 在每个项目中使用与青少年接触四氢大麻酚有关的方案， 在项目中标准化使用化学遗传学方法。核心还将生成，编目， 并分发这些珍贵动物的组织，向科学家开放这一巨大而丰富的资源 世界各地 !

PROJECT SUMMARY: ANIMAL CORE The primary goal of the Animal Core is to provide a standardized, consistent supply of adolescent and adult mice and rats of both sexes treated with THC or its vehicle for use by all ICAL projects. The Core’s trained staff will schedule treatments, behavioral testing and tissue collection, and will administer THC to animals at various ages, treatment regimens, and doses. After treatment and washout, animals will be transferred to the labs of individual ICAL projects (Molecular, Synaptic, and Behavioral). A second objective of the Animal Core is to facilitate consistent and well-validated chemogenetic (DREADD) circuit-control methodologies in ICAL projects. The Core will provide space and instrumentation (stereotaxic frame, virus injection equipment) for viral vector injection, and will train laboratory staff on procedures and protocols to ensure consistent implementation. The core will also maintain and distribute colonies of transgenic Cre driver mouse and rat lines, enabling chemogenetic manipulations of targeted neuronal subtypes including dopamine (TH:Cre or DAT:Cre), as described in individual projects. The third objective of the Animal Core is to establish and maintain an annotated repository of biological material obtained from animals treated in adolescence or adulthood with THC or vehicle. Biomaterial (brain peripheral tissues, fecal matter) from various ICAL activities (pharmacokinetics and pharmacodynamics studies, phenotyping studies, projects) will be collected, catalogued using a computerized bar-coding system and stored at -80oC. An annotated database describing available samples and associated behavioral/physiological data will be created and made available upon request to qualified researchers worldwide. This unique resource, the first in its type, will (i) foster new collaborations between ICAL and diverse research groups around the world; and (ii) open up adolescent cannabinoid research to study by groups unequipped to perform similar treatment regimens themselves. In sum, the Animal Core will be essential to ICAL’s success and broader impact. The Core will develop and implement fully characterized, consistent, and pharmacologically relevant adolescent THC exposure regimens for use in each project, and standardize use of chemogenetic methodologies across projects. The Core will also generate, catalog, and distribute tissue from these valuable animals, opening this large and rich resource to scientists around the world. !