Molecular and Cellular Biology Core

项目摘要： 分子和细胞生物学（MCB）核心设施将通过增加新的 设备和服务，以扩大设施的能力。MCB核心是一个功能齐全的研究 Core目前为CVM用户提供成像和流式细胞术服务。MCB核心设施改进 将专注于扩大DNA测序，CRISPR技术和细胞分选能力。重要的新 堪萨斯州立大学（KSU）为新设备购置和设施维护提供财政支持 和可持续性，并将进一步扩大设施中可用的技术范围， 为当前和未来的研究项目提供前沿技术支持。Philip R. Hardwidge，a 细菌致病机理专家，将担任MCB核心主任。Waithaka Mwangi博士， KSU CVM的流式细胞仪设施和疫苗开发专家，将担任MCB核心副主任， 提供额外的高级领导。MCB对生物信息学和大型DNA处理的核心支持 测序数据集将由KSU生物信息学中心在Sue Brown博士的指导下提供， 生物学分部。DNA测序的专家技术支持将由Doug Marthaler博士提供， 现任KSU兽医诊断实验室DNA测序主任。采用和修订 尖端的CRISPR技术将由格雷格·芬尼根博士提供，在KSU生物化学和 分子生物物理系。将提供额外的技术、具体项目咨询和指导， 由COBRE指导团队、内部和外部咨询委员会成员以及来自 CVM和KSU的生物学部门。一位高级研究助理，迈克海斯，将提供日常 管理设施，并将与项目负责人沟通，以确定具体的，不断变化的需求， 个别项目和核心。Mike Hays在分子和细胞生物学领域拥有丰富的经验， 将使用的生物学方法。核心已经确定了提供先进的 影像技术、下一代DNA测序和CRISPR资源。这些 活动将与动物模型和病理学（AMP）核心紧密结合。AMP核心还将 进行技术升级，包括购置激光捕获显微切割仪器。 两个核心之间将存在显著的协同作用，并将首次在KSU实现以下性能： 单细胞RNA-Seq实验MCB核心将直接支持中心的主要和试点/种子 项目，以及KSU和区域科学网络（RSN）内的用户社区，包括 教职员工博士后和研究生MCB核心设施的显著增强还将 为强大的博士提供良好的培训环境。在KSU的节目。最重要的是，MCB核心 升级将改善KSU生物医学研究的整体基础设施。

Project Summary: The Molecular and Cellular Biology (MCB) Core facility will be modernized and enhanced by adding new equipment and services to expand the capabilities of the facility. The MCB Core is a fully functional research core that currently provides imaging and flow cytometry services to CVM users. MCB Core facility improvements will focus on expanding the DNA sequencing, CRISPR technology, and cell sorting capabilities. Significant new financial support from Kansas State University (KSU) for new equipment acquisition and facility maintenance and sustainability has been obtained and will further expand the range of technologies available in the facility, providing cutting-edge technological support for current and future research projects. Dr. Philip R. Hardwidge, a bacterial pathogenesis expert, will serve as MCB Core director. Dr. Waithaka Mwangi, the current director of KSU CVM's flow cytometry facility and a vaccine development expert, will serve as MCB Core associate director, providing additional senior leadership. MCB Core support for bioinformatics and handling of large DNA sequencing datasets will be provided by the KSU Bioinformatics Center, under the direction of Dr. Sue Brown, Division of Biology. Expert technical support for DNA sequencing will be provided by Dr. Doug Marthaler, the current director of DNA sequencing in the KSU Veterinary Diagnostic Laboratory. Implementation and refinement of cutting-edge CRISPR technology will be provided by Dr. Greg Finnigan, in the KSU Biochemistry and Molecular Biophysics Department. Additional technological, project-specific advice and mentoring will be provided by the COBRE mentoring team, internal and external advisory committee members, and members from the CVM and Division of Biology at KSU. A senior research associate, Mike Hays, will provide day-to-day management of the facility, and will communicate with project leaders to identify specific, evolving needs for individual projects and the Core. Mike Hays possesses significant experience in the molecular and cellular biology methodologies that will be utilized. The Core has already established the feasibility of providing advanced imaging technology, next-generation DNA sequencing and CRISPR resources to junior project leaders. These activities will be tightly integrated with the Animal Model and Pathology (AMP) Core. The AMP core will also undergo technological upgrades, including the acquisition of a laser capture microdissection instrument. Significant synergy will exist between the two cores and will allow for the first time at KSU the performance of single-cell RNA-Seq experiments. The MCB Core will directly support the Center's primary and pilot/seed projects, as well as the community of users within KSU and the regional scientific network (RSN), including faculty, staff, postdocs, and graduate students. The significant enhancement of the MCB Core facilities will also provide an excellent training environment for the robust Ph.D. program at KSU. Most importantly, MCB Core upgrades will improve the overall infrastructure for biomedical research at KSU.