Core D: In Vitro Models and Cell Culture Core-Li

核心D -体外模型和细胞培养核心 项目摘要 体外模型和细胞培养核心继续作为所有项目的资源， 的PPG。它为每个项目提供了相关的细胞培养物，并提供了细胞， 组织、资源和专业知识提供给世界各地的合作者。下面列出的是一些 核心对PPG的贡献。进度报告出版物清单在本报告的末尾。 应用程序. 1.猪、人和小鼠上皮细胞体外模型的产生。 a.来自肺不同区域的猪气道上皮细胞的体外模型。 B.用于比较研究的人和小鼠气道上皮细胞的体外模型。 2.猪和人上皮细胞研究的新方法和模型的开发。 a.开发分离和扩增猪和人气道上皮细胞的方法。 B.开发分离和培养原代猪和人气道上皮细胞的方法。 C.建立猪小气道上皮原代分离培养方法 航空公司. D.开发方法以维持离体气管上皮和肺切片的外植体。 e.建立猪胆囊类器官的培养方法。 3.体外模型和天然上皮细胞的表征。 a.生物电分析。 B.形态学评价。 C.基因表达谱，mRNA和microRNA。 D.气道表面液体（ASL）pH测定。 4.猪成纤维细胞的扩增和保存。 5.教授爱荷华州大学和其他机构的研究人员如何成功地 实施我们的体外模型系统的方法。 6.组织采集、细胞培养和表型相关记录保存。 7.获得所有必要的批准，并保持细胞和动物研究的准确记录， 监管委员会。 8.向其他机构的研究者提供细胞和组织。 9.为PPG的研究者生产培养细胞。 这个核心为企业提供了一个高度积极性和技术先进的工作人员的资源， PPG研究者的优势。因此，它允许各个项目的调查人员 将其独特的专业知识集中在与CF高度相关的问题上。

CORE D – IN VITRO MODELS AND CELL CULTURE CORE PROJECT SUMMARY The In Vitro Models and Cell Culture Core continues to function as a resource for all projects in the PPG. It has provided relevant cell cultures to each of the projects and has provided cells, tissues, resources, and expertise to collaborators around the world. Listed below are some of the contributions of the Core to the PPG. The progress report publication list is at the end of this application. 1. Generation of in vitro models of pig, human, and mouse epithelia. a. In vitro models of pig airway epithelia from distinct regions of the lung. b. In vitro models of human and mouse airway epithelia for comparative studies. 2. Development of new methods and models for the study of pig and human epithelia. a. Develop methods to isolate and expand pig and human airway epithelia. b. Develop methods to isolate and culture primary pig and human airway epithelia. c. Develop methods to isolate and culture primary pig airway epithelia from small airways. d. Develop methods to maintain explants of tracheal epithelia and lung slices ex vivo. e. Develop methods to culture pig gallbladder organoids. 3. Characterization of in vitro models and native epithelia. a. Bioelectric analyses. b. Morphologic evaluation. c. Gene expression profiles, mRNA and microRNA. d. Airway surface liquid (ASL) pH determination. 4. Amplification and storage of pig fibroblasts. 5. Teach investigators at the University of Iowa and other institutions how to successfully implement the methods for our in vitro model systems. 6. Record keeping relevant to tissue acquisition, cell cultures, and phenotypes. 7. Obtain all required approvals and maintain accurate records for cell and animal studies for regulatory committees. 8. Provision of cells and tissues to investigators at other institutions. 9. Production of cultured cells for investigators in the PPG. This Core offers the resources of a highly motivated and technically sophisticated staff to the benefit of the PPG investigators. As a result, it allows investigators of the individual projects to focus their unique expertise on questions of high relevance to CF.