Acoustically-driven optical-interferometric microscope for cell characterization

摘要 该提案的目标是开发第一种声学驱动的定量相位显微镜（QPM）流变学 系统机械力在生理学和病理学中起着重要作用。机械、电气和化学 这些现象支配细胞系统中的所有过程，例如信号传导、分化、迁移和凋亡。蜂窝 力学在正常的生理过程中起着重要的作用，但也许更重要的是在许多不正常的生理过程中， 病理过程。已证明机械环境调节干细胞分化;胚胎 发展是由许多机械线索引导的。在病理过程中，红细胞膜的硬化是一种 镰状细胞病和疟疾患者血管闭塞危象的驱动因素。机械力失调会导致 心肌细胞肥大可导致心脏性猝死，最常见于年轻患者。炎症 已知受机械因素调节，并且与治疗慢性伤口的困难有关。的刚度 细胞外基质环境在调节癌症进展和细胞/组织的测量中是重要的 已经提出机械性能作为在癌症手术期间识别切除边缘的方法。 机械药理学也已知调节细胞药物反应。细胞生物力学的重要性在于 然而，在生物学和医学中研究细胞尺度机械因素的能力受到限制， 可用的测量工具。

Abstract The goal of this proposal to develop a first of its kind acoustically driven quantitative phase microscopy (QPM) rheology system. Mechanical forces play an important role in physiology and pathology. Mechanical, electrical, and chemical phenomena govern all processes in cellular systems such as signaling, differentiation, migration, and apoptosis. Cellular mechanics play important roles in normal physiological processes but perhaps more importantly in numerous abnormal pathological processes. Mechanical environment have been demonstrated to regulate stem cell differentiation; embryo development is guided by many mechanical clues. In pathological processes, stiffening of red blood cell membrane is a factor in driving vaso-occlusive crisis in sickle cell disease and malaria patients. Mechanical forces dysregulation can lead to hypertrophy of cardiomyocytes that can cause sudden cardiac death, most commonly in young patients. Inflammation is known to be regulated by mechanical factors and is related to difficulties in treating chronic wounds. The stiffness of extracellular matrix environment is important in regulating cancer progression and the measurement of cell/tissue mechanical properties has been proposed as a way to identify resection margins during cancer surgery. Mechanopharmacology is also known to modulate cellular drug responses. The importance of cellular biomechanics is well recognized; however, the ability to investigate cellular scale mechanical factors in biology and medicine is limited by the available measurement tools.