Core 3 - Immunobiology Core

摘要-核心3-免疫生物学核心 全世界约有4000万人感染艾滋病毒/艾滋病；然而，保护性疫苗或有效的 尽管进行了40年的紧张研究，但治愈方法仍然难以捉摸。HIV-1通过其自身的功能逃避免疫系统 在感染和复制过程中结构的快速演变。杜克大学艾滋病毒结构生物学中心将致力于 HIV-1包膜蛋白进化的结构研究以阐明结构-功能机制 用于病毒进入、B细胞和T细胞的激活，以及抗逆转录病毒治疗后病毒的反弹。免疫生物学 CORE(CORE 3)汇集了一支在细胞和分子方面拥有广泛专业知识的研究团队 为免疫学提供全面、集中的免疫检测服务，支持三方的努力 项目和其他核心。免疫生物学核心的总体目标是：1)支持项目1、2、 3用免疫分析和生化方法评估细胞系中的B细胞受体信号和敲打- 在小鼠中，2)提供作为B细胞激活读出的MHCII上的肽呈递的测量方法， 和3)支持项目3，从艾滋病毒感染者身上进行单个B细胞分选以进行康复和 Env特异性抗体的表达。免疫生物学核心由设施、技术和服务组成 在杜克人类疫苗研究所凯恩博士(核心领导)的细胞免疫学实验室里 (DHVI)和Borrow博士(核心联席主任)在牛津大学的生物分子相互作用实验室工作。 桑德斯博士(DHVI)的蛋白质生产实验室DVHI的Alam(核心联席董事)和DHVI Flow 细胞仪设备。 除了与个别项目的互动外，免疫生物学核心还将直接与 发展核心。核心人员将与学员在板凳上携手合作，讲授 分析，强调稳健的实验设计、适当的技术和适当的分析 实验结果。根据需要，核心将为受训人员举办小组讲习班，重点是 与核心活动相关的免疫学主题或方法。 免疫生物学核心提供的化验和服务对研究项目的成功至关重要。 免疫分析的提供将提供对B抗原诱导的信号传播的关键见解 B细胞表膜上的细胞受体相互作用及HIV+中新的Env抗体的分离 这些主题将使人们能够深入了解与抑制病毒库有关的结构。此外， 在这个核心中开发的方法和试剂将提供给研究界用于 在基础和应用结构免疫学研究中的应用。

Abstract – Core 3 – Immunobiology Core Approximately 40 million people worldwide are living with HIV/AIDS; however, a protective vaccine or functional cure remain elusive despite four decades of intense research. HIV-1 evades the immune system through its rapid structural evolution during infection and replication. The Duke Center for HIV Structural Biology will pursue structural studies of the evolution of the HIV-1 Envelope (Env) protein to elucidate structure-function mechanisms for viral entry, B-cell and T-cell activation, and viral rebound after antiretroviral therapy ART. The Immunobiology Core (Core 3) brings together a team of investigators with extensive expertise in cellular and molecular immunology to provide comprehensive, centralized immunoassay services to support the efforts of the three projects and the other cores. The overall objectives of the Immunobiology Core are to 1) support Projects 1, 2, and 3 with immunoassays and biochemical protocols to evaluate B cell receptor signaling in cell lines and knock- in mice, 2) provide assays for measurements of peptide presentation on MHCII as a read-out of B cell activation, and 3) support Project 3 by performing single B cell sorting from HIV-infected subjects for recovery and expression of Env-specific antibodies. The Immunobiology Core comprises facilities, technologies, and services housed in the cellular immunology laboratories of Dr. Cain (Core Lead) at the Duke Human Vaccine Institute (DHVI) and Dr. Borrow (Core Co-director) at Oxford University, the biomolecular interaction laboratory of Dr. Alam (Core Co-director) at DVHI, the protein production laboratory of Dr. Saunders (DHVI), and the DHVI Flow Cytometry Facility. In addition to interactions with individual projects, the Immunobiology Core will also directly interface with the Developmental Core. Core personnel will work hand-in-hand with trainees at the bench for the teaching of assays, with emphasis on robust experimental design, proper technique, and appropriate analysis of experimental results. As needed, the Core will host small-group workshops for trainees that focus on immunological topics or methodologies related to Core activities. The assays and services provided by the Immunobiology Core are critical to the success of the research projects. The provision of immunoassays will provide key insights into the propagation of signals induced by antigen-B cell receptor interactions at the B cell surface membrane, and the isolation of novel Env antibodies from HIV+ subjects will enable structural insights pertaining to suppression of the viral reservoir. Moreover, the methodologies and reagents developed in this Core will be made available to the research community for application in basic and applied structural immunology research.