Understanding the role of the frontal cortex during cognitive flexibility in Fmr1 knock outs

项目总结 自闭症谱系障碍(Asd)是一种神经发育障碍，个体在 认知灵活性。目的：本提案将从Fmr1 KOS(脆性X模型)的额叶皮质记录 在认知灵活性任务中)，并评估KO内的神经过度兴奋如何影响 反转学习和注意力转移。方法：对FMR1KO和野生型大鼠进行分类训练。 操作室中触摸屏的感知任务。单单位神经生理学和局域场电位 将在任务期间从额叶皮质(包括前扣带回)收集。重要的是要 了解KO内神经功能的改变如何影响认知功能和学习。这个项目将 比较可能的锥体神经元和中间神经元的神经活动，以检查不同的功能 认知力。检查KOS和WT之间在神经功能上的这些差异将增强我们的 了解大脑皮层兴奋性如何影响认知。局部场势可以提供以下模板 神经特征，在未来的研究中可以与来自人类的脑电数据进行比较 ASD或脆性X。

PROJECT SUMMARY Autism spectrum disorder (ASD) is a neurodevelopmental disorder where individuals exhibit deficits in cognitive flexibility. Purpose: This proposal will record from the frontal cortex of Fmr1 KOs (model of Fragile X syndrome) during cognitive flexibility tasks and assess how neural hyperexcitability within the KOs impacts reversal learning and shifts of attention. Methods: Fmr1 KOs and wildtype rats will be trained on categorical perception tasks with touchscreens in operant chambers. Single-unit neurophysiology and local field potentials will be collected from the frontal cortex (including the anterior cingulate) during the tasks. It is important to understand how altered neural function within the KOs impacts cognitive function and learning. This project will compare neural activity from putative pyramidal and interneurons to examine differential functions during cognition. Examining these differences in neural function between KOs and WT will enhance our understanding of how cortical excitability impacts cognition. The local field potentials can provide templates of neural signatures that in future studies can be compared to electroencephalography data from humans with ASD or Fragile X.