Investigating the effects of adolescent binge drinking on prelimbic somatostatin circuitry

项目摘要 青少年时期的酗酒已被确定为酒精使用障碍（AUD）的潜在危险因素 在成年期。尽管如此，青少年饮酒所改变的精确神经元群体， 可能有助于成年饮酒的变化，尚未完全表征。生长抑素（SST）- 在动物模型中，成年人狂饮改变了前边缘（PL）皮质中的表达神经元， 操纵这些神经元的活动可以减少酗酒。本提案的目的是确定 SST神经元在青少年酗酒以及成年期酒精浓度升高后的作用 青少年暴露后的消费。第一组实验将使用全细胞膜片钳 电生理学和纤维光度学来了解青少年饮酒如何影响PL中的SST神经元 皮层第二组实验将使用行为和化学遗传操作来确定因果关系。 SST神经元在青少年狂饮后成年期饮酒量变化中的作用

PROJECT ABSTRACT Binge drinking during adolescence has been identified as a potential risk factor for alcohol use disorder (AUD) in adulthood. Despite this, the precise neuronal populations which are altered by adolescent drinking, and which may contribute to changes in adulthood drinking, have not been fully characterized. Somatostatin (SST)- expressing neurons in the prelimbic (PL) cortex are altered by adult binge drinking in animals models, and manipulating the activity of these neurons can reduce binge drinking. The goal of this proposal is to determine the role of SST neurons following adolescent binge drinking, as well as during escalated adulthood alcohol consumption following adolescent exposure. The first set of experiments will use whole-cell patch-clamp electrophysiology and fiber photometry to understand how adolescent alcohol use affects SST neurons in the PL cortex. The second set of experiments will use behavioral and chemogenetic manipulations to identify a causal role for SST neurons in changes in adulthood alcohol consumption after adolescent binge drinking.