Investigating the effects of adolescent binge drinking on prelimbic somatostatin circuitry

项目摘要 青春期酗酒已被确认为酒精使用障碍(AUD)的潜在危险因素 在成年后。尽管如此，青少年饮酒改变的准确神经元群，以及 可能对成年后饮酒的变化有贡献，还没有完全的特征。生长抑素(SST)- 在动物模型中，成年酗酒改变了前额叶(PL)皮层的表达神经元，并且 控制这些神经元的活动可以减少酗酒。这项提案的目标是确定 青少年酗酒后以及成年期酗酒后SST神经元的作用 青春期暴露后的消费。第一组实验将使用全细胞膜片钳 电生理学和纤维光度法了解青少年饮酒如何影响PL中的SST神经元 大脑皮层。第二组实验将使用行为和化学遗传操作来确定原因 SST神经元在青少年酗酒后成人饮酒变化中的作用。

PROJECT ABSTRACT Binge drinking during adolescence has been identified as a potential risk factor for alcohol use disorder (AUD) in adulthood. Despite this, the precise neuronal populations which are altered by adolescent drinking, and which may contribute to changes in adulthood drinking, have not been fully characterized. Somatostatin (SST)- expressing neurons in the prelimbic (PL) cortex are altered by adult binge drinking in animals models, and manipulating the activity of these neurons can reduce binge drinking. The goal of this proposal is to determine the role of SST neurons following adolescent binge drinking, as well as during escalated adulthood alcohol consumption following adolescent exposure. The first set of experiments will use whole-cell patch-clamp electrophysiology and fiber photometry to understand how adolescent alcohol use affects SST neurons in the PL cortex. The second set of experiments will use behavioral and chemogenetic manipulations to identify a causal role for SST neurons in changes in adulthood alcohol consumption after adolescent binge drinking.