Laboratory Core

抽象实验室岩心 YCSTP的目标是减少烟草使用和成瘾。通过更好地了解烟草产品如何 成分，如冷却剂、调味剂、甜味剂、尼古丁来源(天然与合成)和尼古丁 对映体(S与R-)的比例影响上诉，我们的目的是告知FDA尼古丁成瘾的风险和 伤害。对研究结果的最佳解释要求关键化学成分准确 在烟草产品和研究参与者的生物样本中进行了量化。的主要目标是 实验室的核心是提供准确、可重现和及时的表征和 量化这些组成部分，以支持中心的项目和试点以及 在必要时创建自定义电子液体。核心将通过追求五个具体目标来实现其目标。(1) 识别和准确量化烟草产品及其产生的蒸气中的化学物质，例如 尼古丁(包括R-和S对映体)、合成冷却剂和薄荷脑、香料、甜味剂等 感兴趣的复合体。(2)对接触和危害产生客观和定量的估计， 通过测量生物样品中的以下生物标记物来检测烟草产品：尼古丁、可替宁、3-羟基 可替宁、总尼古丁当量、尼古丁、薄荷醇葡萄糖醛酸苷和NNAL(4-(甲基亚硝氨基)-1-(3- 1-丁醇)。(3)维护烟草产品成分数据库，如调味剂、冷却剂、 还有甜味剂。(4)为正在进行的研究创造定制的电子液体，而商业产品不是 可因市场变化而提供。(5)保存从研究中获得的生物样本的资料库 参与者。核心还将能够根据不断变化的需求建立其他分析 中间。在过去的9年里，这个核心显著地提升了烟草产品的知识 成分，包括新的成分，特别是这些成分如何导致成瘾和 伤害。核心将是成功的，因为：(A)目标1和目标2所需的许多化学分析是 经过验证并可立即使用，(B)核心在检测新型烟草方面有广泛的记录 (C)核心拥有必要的专门知识、设施和设备，以建立 及时进行新的分析，(D)数据库(目标3)和生物储存库(目标5)已经到位并可以 易于扩展，以容纳从新项目获得的数据和样本；和(E)核心 有创建定制电子液体的经验(目标4)。通过可靠地向青年教师培训计划提供这些服务， 实验室核心将为YCSTP的TCORS 3.0项目的成功做出关键贡献，该项目将 最终帮助告知FDA新产品和成分中尼古丁成瘾的风险。

ABSTRACT – LABORATORY CORE The YCSTP’s goal is to reduce tobacco use and addiction. By understanding better how tobacco product constituents such as coolants, flavorants, sweeteners, nicotine source (natural vs. synthetic) and nicotine enantiomer (S- vs. R-) ratio influence appeal, we aim to inform the FDA about the risk of nicotine addiction and harm. Optimal interpretation of the study outcomes requires that key chemical components are accurately quantified in tobacco products and in biological samples from the study participants. The main objective of the Laboratory Core is to provide accurate, reproducible, and timely characterization and quantification of such components in support of the projects and pilots of the Center, as well as to create custom e-liquids when necessary. The core will attain its objective by pursuing five specific aims. (1) To identify and accurately quantify chemicals in tobacco products and in vapors produced from them, such as nicotine (including R- and S- enantiomers), synthetic coolants and menthol, flavorants, sweeteners, and other compounds of interest. (2) To generate objective and quantitative estimates of exposure to, and harm from, tobacco products by measuring the following biomarkers in biological samples: nicotine, cotinine, 3-hydroxy cotinine, total nicotine equivalents, nicotelline, menthol glucuronide, and NNAL (4-(methylnitrosamino)-1-(3- pyridyl)-1-butanol). (3) To maintain a database of tobacco product constituents, such as flavorants, coolants, and sweeteners. (4) To create custom e-liquids for ongoing studies when commercial products are not available due to market changes. (5) To maintain a repository of biological samples obtained from the study participants. The core will also be able to set up other assays, as mandated by the evolving needs of the center. Over the past 9 years, this core has significantly advanced knowledge about tobacco product constituents, including novel ones, and particularly how these constituents contribute to addiction and harm. The core will be successful because: (a) many of the chemical assays necessary for aims 1 and 2 are validated and ready for immediate use, (b) the core has an extensive track record of detecting novel tobacco product constituents of concern, (c) the core has the necessary expertise, facilities and equipment to set up new assays in a timely fashion, (d) the database (aim 3) and biorepository (aim 5) are already in place and can readily be expanded to accommodate the data and samples obtained from the new projects, and (e) the core has experience in creating custom e-liquids (aim 4). By providing these services reliably to the YCSTP, the Laboratory Core will contribute critically toward the success of YCSTP’s TCORS 3.0 projects, which will ultimately aid in informing the FDA on the risk of nicotine addiction in new products and constituents.