Protein Production and Characterization Core

项目总结(蛋白质生产和表征核心) 蛋白质生产和表征核心(PPCC)为俄克拉荷马眼镜蛇提供研究支持 在结构生物学(OCSB)和更大的生命科学研究界。它是一个最先进的设施，用于 研究人员蛋白质的表达、纯化和生物物理特性。人民政治协商会议生产 在收费的基础上以可靠和高效的方式提供高质量的蛋白质，并提供动手培训 以及设施中提供的蛋白质表达、纯化和表征方面的所有方面的专家建议。 PPCC的工作人员包括核心主任菲利普·伯恩博士和一名研究生助理。政协是 由一个由三名教师用户组成的咨询委员会指导。 PPCC与调查人员和OCSB支持的生物分子结构核心(BSC)密切合作，以 为结晶试验和其他生命科学研究需要生产均一、高纯度的蛋白质。这个 设施配备了在个别研究实验室中经常找不到的仪器，因为 成本和维护费用。最近，用于大分子生物物理表征的仪器被 增加了设施，并促进了服务的扩展，从而增加了用户基础。 在拟议的OCSB第三阶段期间，PPCC将与BSC、OU质谱学、 蛋白质组学和代谢组学核心，以及俄亥俄大学萨缪尔·罗伯茨诺布尔显微镜的冷冻-EM组件 实验室支持研究人员对结构生物学方法学的蛋白质需求。成为一种动力 为生命科学界提供的资源将继续扩大用户基础，并在 大学，为这一核心设施的长期可持续性提供保障。

Project Summary (Protein Production and Characterization Core) The Protein Production and Characterization Core (PPCC) serves as research support for the Oklahoma COBRE in Structural Biology (OCSB) and the greater life science research community. It is a state-of-the-art facility for the expression, purification, and biophysical characterization of proteins for researchers. The PPCC produces high quality proteins in a reliable and efficient manner on a fee-for-service basis and provides hands-on training and expert advice in all aspects of protein expression, purification, and characterization available in the facility. The PPCC staff consists of the Core Director Dr. Philip Bourne and a Graduate Student Assistant. The PPCC is guided by an advisory committee comprised of three faculty users. The PPCC works closely with investigators and the OCSB-supported Biomolecular Structure Core (BSC) to produce homogeneous, highly purified protein for crystallization trials and other life science research needs. The facility is equipped with instrumentation frequently not found within individual research laboratories due to their cost and maintenance. Recently, instrumentation for the biophysical characterization of macromolecules was added to the facility and fostered the expansion of services and resulting increase in the user base. During the proposed Phase 3 of the OCSB, the PPCC will work with the BSC, the OU Mass Spectrometry, Proteomics & Metabolomics Core, and the cryo-EM component of the OU Samuel Roberts Noble Microscopy Laboratory to support researchers in their protein needs for structural biology methodologies. Being a dynamic resource for the life science community will continue to grow the user base and, together with the support of the University, provide for the long-term sustainability of this core facility.