Bruker Atomic Force Microscope NanoWizard® 5

摘要 在这个共享仪器赠款提案中，我们要求资金大大丰富和扩大能力 该大学基于原子力显微镜（AFM）的单分子、细胞和生物材料分析 位于圣安东尼奥的德克萨斯健康科学中心（UT Health SA）的生物分析和单细胞核心（BASIC）。 BASIC及其AFM实验室位于分子医学系，是 研究副总统办公室下属的机构研究核心。拟议的进步 包括从布鲁克公司购买AFM系统Nanowtron 5。该系统将包括一个JPK NanoWizard® V AFM头、带高级版本选项的JPK Vortis 2.1 SPM控制器和JPK HybridStage（200 x200 x200 µm³）安装在Nikon Eclipse Ti 2-U倒置荧光显微镜上，置于 在台式主动隔振系统上的隔音罩。进一步的增强功能包括 微流体系统V2 FluidFM®，JPK Cell Mechanics Package和JPK SideView悬臂保持器，用于 对于单细胞操作是不可或缺的。该系统将由一些重要的AFM支持 硬件、附件、工具和用于仪器控制、数据采集和分析的专用软件。的 BASIC AFM设施已经成功地为大学社区和超越十年提供服务， 关于癌细胞的基本结构和机械数据，包括患者分离的细胞，细胞器， 大分子和生物材料支持众多的出版物，赠款申请和其他相关的 活动在UT健康SA的基础，转化和临床研究的广泛阵列取决于可访问， 经济实惠，可定制的基于AFM的分子和细胞成像技术，包括机械 表型分析、化学成像和力谱分析。已有十年历史的Nanoscope催化剂 效率低下，患者、动物模型和生物材料样品的电流过载，没有空闲时间 学生培训或批判性方法发展。此外，它不能映射医学上重要的组织，类器官， 和宽敞的样品。它也被严格限制在研究 细胞与细胞之间的相互作用对基础和医学研究至关重要。当前设置不提供 单细胞液体注射、取样和操作能力，对于所有测序和联用至关重要 与机械和荧光成像接口的应用。催化剂还显示了一个妥协 分子物体的分辨率与拟议的纳米粒子5相比。Catalyst无法升级， 其备件供应有限。因此，新的拟议设置不仅是必不可少的， 适应当前研究的数量并保持其质量，但也要极大地扩大其 多样性，并提高其价值，这将有利于广泛的生物医学用户与 大圣安东尼奥科学界。

Abstract In this Shared Instrumentation Grant proposal, we request funds to substantially enrich and expand capabilities of atomic force microscopy (AFM) based analysis of the single molecules, cells, and biomaterials at the University of Texas Health Science Center at San Antonio (UT Health SA) in the Bioanalytics and Single-Cell Core (BASiC). BASiC and its AFM laboratory is located in the Department of Molecular Medicine and it is a part of the Institutional Research Core under the Office of the Vice President for Research. The proposed advancement includes a purchase of the AFM system Nanowizard 5 from Bruker. The system will consist of a JPK NanoWizard® V AFM head, JPK Vortis 2.1 SPM Controller with its advanced version option, and a JPK HybridStage (200x200x200 µm³) mounted on a Nikon Eclipse Ti2-U inverted fluorescent microscope placed in an acoustic enclosure on a benchtop active vibration isolation system. Further enhancements include a microfluid system V2 FluidFM®, JPK Cell Mechanics Package and JPK SideView cantilever holder for indispensable for single cell manipulations. The system will be supported by a number of essential AFM hardware, accessories, tools, and specialized software for instruments control, data capture and analysis. The BASiC AFM facility has been successfully serving the University community and beyond for ten years delivering essential structural and mechanical data on cancer cells including patient isolated cells, organelles, macromolecules and biomaterials supporting numerous publications, grant applications and other associated activities. A broad array of basic, translational, and clinical studies at the UT Health SA depends on accessible, affordable, and customizable AFM-based molecular and cellular imaging technologies including mechanical phenotyping, chemical imaging and force spectrometry. The existing ten years old Nanoscope Catalyst is inefficient with the current overload of patient, animal model, and biomaterial samples leaving no free time for students training or critical methods development. Moreover, it cannot map medically crucial tissue, organoid and spacious samples under the physiologically relevant conditions. It is also severely restricted in studies of cell to cell interactions that are crucial for basic and medical studies. The current setup does not provide capabilities for single cell liquid injection, sampling, and manipulation vital for all sequencing and hyphenated applications interfaced with mechanical and fluorescent imaging. The Catalyst also shows a compromised resolution of molecular objects comparing to the proposed Nanowizard 5. The Catalyst cannot be upgraded and availability of its spare parts is limited. Therefore, the new proposed setup is indispensable not only to accommodate the quantity of current research and preserve its quality, but also to enormously expand its diversity and enhance its value that will benefit a broad spectrum of the biomedical users associated with the greater San Antonio scientific community.