Rapid NGS Method for Mapping of the Epitranscriptome

项目总结/摘要 表位转录组学是研究RNA修饰的一门科学，包括170多种天然存在的化学修饰。 对核苷酸的改变。在人类所有类型的RNA中发现了60多种：mRNA，tRNA，rRNA，lncRNA， 还有其他人这些修改是动态的;它们的整体数量在发展和期间发生变化 疾病进展。它们由写入酶安装，由读取蛋白读取，并由擦除器移除 它们具有改变RNA结构和动力学的内在能力。影响翻译 起始和终止、翻译保真度、可变剪接、细胞区室之间的运输，以及 调节RNA降解。RNA阅读器、写入器和擦除器蛋白是有前途的高电流药物靶点 对制药感兴趣。在这个项目中，我们将开发和商业化一种新的方法来检测，识别， 和映射，RNA修饰的多重和高灵敏度-适用于临床样品（例如， 针活检，FFPE样品），其中仅可获得亚纳克量的RNA。这 这项技术将具有重要意义，因为它将提供第一种用于分析和关联的商业方法 使用低样品输入，在整个转录组中的多种RNA修饰类型的变化。

Project Summary/Abstract Epitranscritomics is the study of RNA modifications, which include more than 170 naturally occurring chemical alternations to the nucleotides. More than 60 are found in human RNA of all types: mRNA, tRNA, rRNA, lncRNA, and the others. These modifications are dynamic; their global quantities change in development and during disease progression. They are installed by writer enzymes, read by reader proteins and removed by eraser enzymes, and they have an intrinsic capacity to alter RNA structure and dynamics. They influence translation initiation and termination, translation fidelity, alternative splicing, trafficking between cellular compartments, and regulate RNA degradation. RNA reader, writer and eraser proteins are promising drug targets of high current interest to pharma. In this project, we will develop and commercialize a new approach to detecting, identifying, and mapping, RNA modifications in a multiplex and with high sensitivity—suitable for clinical samples (e.g. needle biopsies, FFPE samples) in which only sub-nanogram quantities of RNA may be available. This technology will be significant because it will provide the first commercial method for profiling and correlating changes of multiple RNA modification types across the entire transcriptome using low sample input.