Pathology & Genomics

项目总结： 综合病理学和基因组学核心为肺癌样本提供最先进的分析服务 从最初的样品处理和处理到显微分析，由经认证的 解剖病理学家，免疫表型分析，分子遗传和基因组分析。这部作品 构成肺癌管理的中坚力量，使诊断和适当的治疗选择成为可能 适用于所有类型的肺癌患者，并使癌症生物学、病因学和 管理层。我们的病理学核心支持了这项工作，这导致了对 肺癌生物学及其相关管理，包括对肺癌的重要性的开创性发现 肺癌中EGFR、ALK、ROS1和MET的突变，其诊断和治疗已成为 这一医学领域的国际护理标准。同样，我们打算继续我们的工作。 为了促进对肺癌的了解和护理，通过支持在此提出的新项目， 结合护理标准和创新的尖端新技术。 在这个孢子提案中，我们将支持每个拟议的项目与护理标准相结合 临床病理服务--如肿瘤的组织学特征、免疫组织化学评估 蛋白质表达，以及肿瘤DNA大规模并行测序--以及创新的新技术。其中 将为该项目部署的创新新技术是定制的等位基因特异性聚合酶链式反应分析 单个融合转录本能够超灵敏地监测非细胞循环中的ALK重排 血浆样品、循环免疫荧光和转录组分同步原位空间表达 参与各种生化途径的多种蛋白质和转录本，以及多路离子束成像 提供对癌内和癌旁免疫细胞渗入的高级别评估。

Project Summary: The Integrated Pathology and Genomics Core provides state-of-the-art analytic services for lung cancer samples spanning the full spectrum from initial sample handling and processing, to microscopic analysis by certified anatomic pathologists, to immunophenotypic analysis, to molecular genetic and genomic analysis. This work constitutes the backbone of lung cancer management, and enables the diagnosis and proper treatment selection for patients with lung cancers of all types, and also enables research into cancer biology, etiology, and management. Our pathology core has supported work that has led to major advances in the understanding of lung cancer biology, and associated management thereof, including seminal discoveries of the importance of mutations in EGFR, ALK, ROS1, and MET in lung cancer, the diagnosis and management of which have become the international standard-of-care in this area of medicine. In the same manner, we intend to continue our work to advance the understanding and care of lung cancer, by supporting the new projects proposed herein, with a combination of standard-of-care and innovative new cutting-edge technologies. In this SPORE proposal, we will support each of the proposed projects with a combination of standard-of-care clinical pathology services - such as histologic characterization of tumors, immunohistochemical assessment of protein expression, and massively parallel sequencing of tumor DNA - and innovative new techniques. Among the innovative new techniques to be deployed for this project are customized allele-specific PCR assays for individual fusion transcripts to enable ultrasensitive monitoring of cell-free circulating ALK rearrangements in plasma samples, cyclic immunofluorescence and transcriptomics for simultaneous in situ spatial expression of multiple proteins and transcripts involved in various biochemical pathways, and multiplexed ion beam imaging to provide high-order assessment of immune cell infiltrates within and adjacent to cancers.