Examining Associations between the Oral Microbiota, Neuroinflammation, and Binge Drinking in Adolescents

项目摘要/摘要 拟议的应用响应了更好地了解微生物组和 青少年饮酒者的神经炎症。早期开始饮酒，特别是酗酒，是 被认为是以后酗酒和吸毒的最重要的风险因素之一 疾病，但可能解释这种脆弱性增加的神经生物学变化并不是很好 明白了。早期酒精诱导的微生物群改变可能导致神经代谢物改变和 反过来又会影响酒精使用的升级。因此，微生物组可能是一个新的研究领域 可通过提供新的治疗目标来帮助青少年AUD预防和干预努力，和/或 诊断和治疗反应生物标志物。因此，本研究旨在调查两者之间的联系 在青少年酗酒的背景下，微生物区系和神经炎症之间的关系。具体来说，这项研究 将依赖于对口腔微生物区16S rRNA测序和磁共振波谱(MRS)数据的分析， 为了确定酗酒青少年是否有更高水平的促炎微生物和MRS 神经炎症的标记物。将这些数据放在一起，无论它们是否符合我们的假设，都将 为神经科学和微生物组提供预防和预防的知情目标和战略奠定基础 对有问题的酒精使用的干预努力。在此F31申请中提出的研究将用作 为促进布里特尼在青少年酒精使用障碍中的职业发展而进行的基本实践培训 神经生物学、神经成像和微生物组，它将产生用于构建NIH F32的数据 申请。

PROJECT SUMMARY/ABSTRACT The proposed application responds to the need for a better understanding of the microbiome and neuroinflammation in adolescent alcohol users. Early initiation of alcohol use, and binge drinking in particular, is considered one of the most important risk factors in the later development of alcohol and substance use disorders, but the neurobiological changes that may account for this increased vulnerability are not well understood. Alcohol-induced microbiome alterations at an early age may lead to neurometabolite alterations and in-turn affect the escalation of alcohol use. Consequently, the microbiome may be a novel area of research that may aid in adolescent AUD prevention and intervention efforts by providing new targets for treatment, and/or diagnostic and therapeutic response biomarkers. The present study therefore aims to investigate associations between the microbiota and neuroinflammation in the context of adolescent binge drinking. Specifically, the study will rely on analysis of oral microbiota 16S rRNA sequencing and magnetic resonance spectroscopy (MRS) data, in order to determine if binge drinking adolescents have higher levels of pro-inflammatory microbes and MRS markers of neuroinflammation. Together these data, regardless of if they are line with our hypotheses, will provide a foundation for neuroscience and microbiome informed targets and strategies for prevention and intervention efforts for problematic alcohol use. The research proposed in this F31 application will serve as essential hands-on training to promote Brittney's career development in adolescent alcohol use disorder neurobiology, neuroimaging, and the microbiome, and it will result in data on which to build an NIH F32 application.